Discovery of a readily heterologously expressed Rubisco from the deep sea with potential for CO2 capture

Bioresources and Bioprocessing

Table 2 Substrates consumption rates, products formation rates, carbon recovery and net flux of NADH in Rubisco-based CO₂ capture model

	Substrates consumption rates and products formation rates^a (mmol gDCW⁻¹ h⁻¹)			Carbon recovery (C atoms)
	197-2021	7002	RPE	197-2021	7002	RPE
Xylose	0.41 ± 0.01	0.67 ± 0.03	0.50 ± 0.11	2.05 ± 0.05	3.35 ± 0.15	2.50 ± 0.55
Glycerol	0.18 ± 0.03	0.28 ± 0.06	0.46 ± 0.07	0.54 ± 0.09	0.84 ± 0.18	1.38 ± 0.21
Uptake ¹³CO₂^b	–	0.05 ± 0.002	0.18 ± 0.04	–	0.05 ± 0.002	0.18 ± 0.04
Lactate	0.57 ± 0.02	0.89 ± 0.03	1.01 ± 0.11	1.71 ± 0.06	2.67 ± 0.09	3.03 ± 0.33
Acetate	0.11 ± 0.01	0.11 ± 0.02	0.18 ± 0.02	0.22 ± 0.02	0.22 ± 0.04	0.36 ± 0.04
Ethanol	0.01 ± 0.002	0.06 ± 0.01	0.06 ± 0.01	0.02 ± 0.004	0.12 ± 0.02	0.12 ± 0.02
Released CO₂^c				0.12 ± 0.01	0.17 ± 0.03	0.24 ± 0.03
Total carbon consumption				2.59 ± 0.14	4.24 ± 0.33	4.06 ± 0.80
Total carbon production				2.07 ± 0.10	3.18 ± 0.18	3.75 ± 0.42
Carbon balance				0.80 ± 0.01	0.75 ± 0.02	0.92 ± 0.08
Net flux of NADH^d				0.56 ± 0.01	0.60 ± 0.03	0.74 ± 0.08

^aCalculated by the detected molar concentration divided by dry cell weight, one unit OD₆₀₀ = 0.3 gDCW/L (Soini et al. 2008), the cell density of samples remained stable around the initial 2 unit OD₆₀₀
^bThe value of X₂ was calculated by Eqs. 1, 2, 3 based on ¹³C-labeled lactate production
^cAssumed that released CO₂ was equal to the summation of the moles of acetate and ethanol in the dissimilation of pyruvate
^dCalculated by the ratio of NADH consumption to NADH production, NADH consumption accompanied the production of ethanol and lactic acid, NADH production was from the reaction of glycerol to glycerone and G3P to 1,3BPG, one molecule of NADH consumed or produced per reaction