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Table 2 Substrates consumption rates, products formation rates, carbon recovery and net flux of NADH in Rubisco-based CO2 capture model

From: Discovery of a readily heterologously expressed Rubisco from the deep sea with potential for CO2 capture

  Substrates consumption rates and products formation ratesa (mmol gDCW−1 h−1) Carbon recovery (C atoms)
  197-2021 7002 RPE 197-2021 7002 RPE
Xylose 0.41 ± 0.01 0.67 ± 0.03 0.50 ± 0.11 2.05 ± 0.05 3.35 ± 0.15 2.50 ± 0.55
Glycerol 0.18 ± 0.03 0.28 ± 0.06 0.46 ± 0.07 0.54 ± 0.09 0.84 ± 0.18 1.38 ± 0.21
Uptake 13CO2b 0.05 ± 0.002 0.18 ± 0.04 0.05 ± 0.002 0.18 ± 0.04
Lactate 0.57 ± 0.02 0.89 ± 0.03 1.01 ± 0.11 1.71 ± 0.06 2.67 ± 0.09 3.03 ± 0.33
Acetate 0.11 ± 0.01 0.11 ± 0.02 0.18 ± 0.02 0.22 ± 0.02 0.22 ± 0.04 0.36 ± 0.04
Ethanol 0.01 ± 0.002 0.06 ± 0.01 0.06 ± 0.01 0.02 ± 0.004 0.12 ± 0.02 0.12 ± 0.02
Released CO2c    0.12 ± 0.01 0.17 ± 0.03 0.24 ± 0.03
Total carbon consumption    2.59 ± 0.14 4.24 ± 0.33 4.06 ± 0.80
Total carbon production    2.07 ± 0.10 3.18 ± 0.18 3.75 ± 0.42
Carbon balance    0.80 ± 0.01 0.75 ± 0.02 0.92 ± 0.08
Net flux of NADHd    0.56 ± 0.01 0.60 ± 0.03 0.74 ± 0.08
  1. aCalculated by the detected molar concentration divided by dry cell weight, one unit OD600 = 0.3 gDCW/L (Soini et al. 2008), the cell density of samples remained stable around the initial 2 unit OD600
  2. bThe value of X2 was calculated by Eqs. 1, 2, 3 based on 13C-labeled lactate production
  3. cAssumed that released CO2 was equal to the summation of the moles of acetate and ethanol in the dissimilation of pyruvate
  4. dCalculated by the ratio of NADH consumption to NADH production, NADH consumption accompanied the production of ethanol and lactic acid, NADH production was from the reaction of glycerol to glycerone and G3P to 1,3BPG, one molecule of NADH consumed or produced per reaction