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Table 2 Composition of hydrolysates

From: Applications and analysis of hydrolysates in animal cell culture

References for amino acids Plant-based hydrolysate Animal-based hydrolysate Microorganism-based hydrolysate
Rice bran protein %(w/w) Rapeseed %(w/w) Soy protein %(w/w) Cotton seed %(w/w) Atlantic salmon %(w/w) Chicken hydrolysate %(w/w) Yeast %(w/w)
Amagliani et al. (2017) He et al. (2013) Gorissen et al. (2018) Cheng et al. (2020) Harnedy et al. (2018), Nesse et al. (2014) Wu et al. (2020) Wasserman (1961), Podpora et al. (2016)
Ala 2.25 3.26 2.8 4.1 3.9 5.49 2.67
Arg 2.55 5.23 4.8 13.2 2.85 5.78 1.74
Asn 6.4 4.5
Asp 2.91 9.8 8.38 3.69
Cys 0.46 1.13 0.2 0.38 0.61 0.47
Gln 12.63 6.83
Glu 4.39 12.4 22.1 13.89 5.77
Gly 1.83 3.82 2.7 4.3 4.2 4.03 1.88
His 0.86 2.58 1.5 3.5 1.2 3.24 0.87
Ile 1.04 2.51 1.9 2.33 3.92 1.79
Leu 1.98 5.11 5 3.6 4.13 7.39 2.61
Lys 1.61 4.23 3.4 6.8 3.98 8.12 2.64
Met 0.59 NA 0.3 3.5 1.43 1.87 0.57
Phe 1.25 3.06 3.2 1.2 2.18 3.01 1.75
Pro 1.29 4.34 3.3 7.4 2.55 2.71 2.42
Ser 1.38 3.54 3.4 3.6 2.18 3.35 1.82
Thr 1.31 NA 2.3 5.3 2.33 3.9 1.65
Trp 0.44 0.94 0.6 0.79 0.49
Tyr 1.06 2.7 2.2 3.2 1.88 2.88 1.38
Val 1.64 3.51 2.2 3 3.08 4.15 2.19
Fat/lipids (reference) 2.73 7 17.8 (Bertrand et al. 2005) 0.5 8.38 0.2
References for vitamins Champagne et al. (2004)   University of Rochester Medical Center (2021)   Nesse et al. (2014) Pinto e Silva et al. (2008) Wasserman (1961)
A (retinoid) 0 to 0.00257
B1 (thiamine) 0.00857 to 0.0171 4.22 × 10–8 1.8 × 10–5 0.00007 0.0104 to 0.025
B2 (riboflavin) 0.00128 to 0.00307 2.53 × 10–8 1.575 × 10–5 0.0025 to 0.008
B3 (niacin) 0.191 to 0.356 3.46 × 10–8 3.15 × 10–4 0.03 to 0.0627
B6 (pyridoxine) 0.00643 to 0.02 2.53 × 10–8 5.025 × 10–5 0.0023 to 0.004
B9 (folic acid) 0.000286 to 0.001 1.425 × 10–5 0.0019 to 0.003
B12 (cobalamin) 0 to 0.00257 1.2 × 10–5 0.00008
Biotin 0.000143 to 0.000357 0.00011
C (ascorbic acid) 0.0186 to 0.0929 4.275 × 10–3 0.0475
Choline 0.657 to 1
E (alpha–tocopherol) 0 to 2.86 × 10–6
Inositol 2.86 to 5.71
p–Aminobenzoic acid 0.000464 0.0015 to 0.004
Pantothenic acid 0.0143 to 0.0436 1.69 × 10–8 0.0072 to 0.0086
  1. “–” indicates no available data