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Table 3 Hydrolysates improve the growth and productivity of cell lines

From: Applications and analysis of hydrolysates in animal cell culture

Hydrolysate used Cell type Purpose Effects References
Plant peptones CHO-320 (a CHO K1 clone) Human interferon-gamma production cells Improves cultivation and productivity Burteau et al. (2003)
Yeast hydrolysate CHO Human beta-interferon production Higher productivity with equivalent glycosylation Spearman et al. (2014)
Yeast hydrolysate rCHO (recombinant CHO) Human thrombopoietin (hTPO) production Higher cell growth and hTPO production by 11.5 times Sung et al. (2004), Mosser et al. (2013)
Rice protein hydrolysate CHO-320 Interferon-gamma production Protection against oxidation stress from hydrogen peroxide Mols et al. (2004)
Rice protein hydrolysate Human HepG cells Cell-based bioassays for food antioxidant activity analysis Protection against oxidation stress from hydrogen peroxide Zhang et al. (2016)
Soy peptones (CoyA2SC, SoyE-110) CHO DG44 Testing cell model Improved cell production Davami et al. (2015)
Yeast, soybean and Ex-Cell CD (chemically defined hydrolysate replacement) CHO mAb production Increased mAb titer and specific productivity Ho et al. (2016)
Wheat hydrolysate Improved cell viability but not productivity
Yeast and soybean hydrolysates Affected the distribution of galactosylated glycans
Ex-Cell CD Maintained glycan profile
Yeast extract CHO Fc-fusion protein production Improved Fc-fusion protein productivity Hu et al. (2018)
Yeast extract and peptones CHO-AMW Recombinant lgG1 anti-human RhD mAb production Improved maximal cell density by 70% & IgG production by 180% Mosser et al. (2013)
Silk sericin hydrolysate (from the waste of silk processing) CHO and HeLa cells Testing cell model Improved cell growth and proliferation Zhang et al. (2019b)
Chlorella vulgaris extract CHO-K1 and MSC Protein expression and steam cell phenotype in MSC Promoted the growth of CHO and MSC and increased in protein expression in CHO Ng et al. (2020)
Rapeseed cakes CHO-C5 Testing model Improved growth of CHO at hydrolysis degree of 5 to 30% Chabanon et al. (2007)
Rapeseed Insect Sf9 cells expression of recombinant proteins from baculovirus expression system Promoted the growth of insect Sf9 insect cells in serum-free media Deparis et al. (2003)
Lactalbumin hydrolysate Mouse Swiss 3T3 cells DNA stimulation synthesis Enhances release of plasminogen activator and stimulates DNA synthesis of mouse Swiss 3T3 cells Chou et al. (1979)
Kabuli type chickpea Monocytic THP-1 cells Tools for investigating monocyte structure and function in both health and disease Supports the growth of THP-1 cell line in the absence of serum Girón-Calle et al. (2008)
Wheat gluten protein hydrolysates Primary human monocytes Involved in inflammatory and anti-inflammatory processes during an immune response Leads to potent anti-inflammatory and atheroprotective properties Montserrat-de la Paz et al. (2020)
Tryptone N1 HEK293 EBNA cell line Production of Tie2 ectodomain Leads to a twofold increase in volumetric SEAP (secreted alkaline phosphatase) productivity Pham et al. (2005)
Bonito hydrolysate CHO Anti-human IL-6 receptor antibody production Leads to a 2.2-fold increase in antibody concentration after 7 days of fed-batch culture Goto et al. (2008)