Table 3 Hydrolysates improve the growth and productivity of cell lines
From: Applications and analysis of hydrolysates in animal cell culture
Hydrolysate used | Cell type | Purpose | Effects | References |
|---|---|---|---|---|
Plant peptones | CHO-320 (a CHO K1 clone) | Human interferon-gamma production cells | Improves cultivation and productivity | Burteau et al. (2003) |
Yeast hydrolysate | CHO | Human beta-interferon production | Higher productivity with equivalent glycosylation | Spearman et al. (2014) |
Yeast hydrolysate | rCHO (recombinant CHO) | Human thrombopoietin (hTPO) production | Higher cell growth and hTPO production by 11.5 times | |
Rice protein hydrolysate | CHO-320 | Interferon-gamma production | Protection against oxidation stress from hydrogen peroxide | Mols et al. (2004) |
Rice protein hydrolysate | Human HepG cells | Cell-based bioassays for food antioxidant activity analysis | Protection against oxidation stress from hydrogen peroxide | Zhang et al. (2016) |
Soy peptones (CoyA2SC, SoyE-110) | CHO DG44 | Testing cell model | Improved cell production | Davami et al. (2015) |
Yeast, soybean and Ex-Cell CD (chemically defined hydrolysate replacement) | CHO | mAb production | Increased mAb titer and specific productivity | Ho et al. (2016) |
Wheat hydrolysate | Improved cell viability but not productivity | |||
Yeast and soybean hydrolysates | Affected the distribution of galactosylated glycans | |||
Ex-Cell CD | Maintained glycan profile | |||
Yeast extract | CHO | Fc-fusion protein production | Improved Fc-fusion protein productivity | Hu et al. (2018) |
Yeast extract and peptones | CHO-AMW | Recombinant lgG1 anti-human RhD mAb production | Improved maximal cell density by 70% & IgG production by 180% | Mosser et al. (2013) |
Silk sericin hydrolysate (from the waste of silk processing) | CHO and HeLa cells | Testing cell model | Improved cell growth and proliferation | Zhang et al. (2019b) |
Chlorella vulgaris extract | CHO-K1 and MSC | Protein expression and steam cell phenotype in MSC | Promoted the growth of CHO and MSC and increased in protein expression in CHO | Ng et al. (2020) |
Rapeseed cakes | CHO-C5 | Testing model | Improved growth of CHO at hydrolysis degree of 5 to 30% | Chabanon et al. (2007) |
Rapeseed | Insect Sf9 cells | expression of recombinant proteins from baculovirus expression system | Promoted the growth of insect Sf9 insect cells in serum-free media | Deparis et al. (2003) |
Lactalbumin hydrolysate | Mouse Swiss 3T3 cells | DNA stimulation synthesis | Enhances release of plasminogen activator and stimulates DNA synthesis of mouse Swiss 3T3 cells | Chou et al. (1979) |
Kabuli type chickpea | Monocytic THP-1 cells | Tools for investigating monocyte structure and function in both health and disease | Supports the growth of THP-1 cell line in the absence of serum | Girón-Calle et al. (2008) |
Wheat gluten protein hydrolysates | Primary human monocytes | Involved in inflammatory and anti-inflammatory processes during an immune response | Leads to potent anti-inflammatory and atheroprotective properties | Montserrat-de la Paz et al. (2020) |
Tryptone N1 | HEK293 EBNA cell line | Production of Tie2 ectodomain | Leads to a twofold increase in volumetric SEAP (secreted alkaline phosphatase) productivity | Pham et al. (2005) |
Bonito hydrolysate | CHO | Anti-human IL-6 receptor antibody production | Leads to a 2.2-fold increase in antibody concentration after 7Â days of fed-batch culture | Goto et al. (2008) |