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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: One-pot synthesis of (R)- and (S)-phenylglycinol from bio-based l-phenylalanine by an artificial biocatalytic cascade

Fig. 1

Construction of recombinant E. coli cells co-expression of six enzymes for conversion of L-PA 1 to 2-HAP 6. A Construction of recombinant E. coli cells co-expression of six enzymes based on different plasmid configurations. B SDS-PAGE analysis of recombinant E. coli cells. M: marker; lane 1: cell-free extract of E. coli (RFP-DEA-CGS), lane 2: cell-free extract of E. coli (CFP-DES-RGA); lane 3: cell-free extract of E. coli (DFP-CAS-REG). C Activities of three different recombinant E. coli cells for conversion of L-PA 1 to 2-HAP 6. D Cell growth and specific activity of E. coli (RFP-DEA-CGS) for conversion of L-PA 1 to 2-HAP 6

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