Fig. 3From: One-pot synthesis of (R)- and (S)-phenylglycinol from bio-based l-phenylalanine by an artificial biocatalytic cascadeConstruction of recombinant E. coli cells for conversion of 2-HAP 6 to (R)-phenylglycinol 7. A Construction of recombinant E. coli cells co-expression of three enzymes (BMTA, AlaDH and GDH) based on different plasmid configurations. B SDS-PAGE analysis of recombinant E. coli cells. M: marker; lane 1: cell-free extract of E. coli (EB-DGA), lane 2: cell-free extract of E. coli (EB-DG-CA). C Activities of two different recombinant E. coli cells for conversion of 2-HAP 6 to (R)-phenylglycinol 7Back to article page