Fig. 5

Time course of the cascade reaction for the synthesis of (S)-7 from L-PA 1. A Time course of the cascade reaction for conversion of 10–30 mM L-PA 1 to (S)-7; B time course of the cascade reaction for intermediate produced from L-PA 1. Reaction conditions (5 mL): 100 mM phosphate buffer (pH 7.5), 10–30 mM L-PA, 10 mM glucose, 20 g cdw/L E. coli (RFP-DEA-CGS), 15 g cdw/L E. coli (MVTA), 30 mM (R)-MBA and 0.1 mM PLP, at 25 °C. All biotransformations were performed in duplicate and conversion was determined by GC, error limit: < 2% of the state values