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Fig. 7 | Bioresources and Bioprocessing

Fig. 7

From: One-pot synthesis of (R)- and (S)-phenylglycinol from bio-based l-phenylalanine by an artificial biocatalytic cascade

Fig. 7

Time course of the cascade reaction for the synthesis of (R)-7 from L-PA 1. A Time course of the cascade reaction for conversion of 10–30 mM L-PA to (R)-7; B time course of the cascade reaction for intermediate produced from L-PA 1. Reaction conditions (5 mL): 100 mM phosphate buffer (pH 7.5), 10–30 mM L-PA, 20 mM glucose, 15 g cdw/L E. coli (RFP-DEA-CGS), 20 g cdw/L E. coli (EB-DGA), 400 mM L-ala, 150 mM NH3/NH4Cl, 0.1 mM PLP, at 25 °C. All biotransformations were performed in duplicate and conversion was determined by GC, error limit: < 2% of the state values

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