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Scheme 1 | Bioresources and Bioprocessing

Scheme 1

From: One-pot synthesis of (R)- and (S)-phenylglycinol from bio-based l-phenylalanine by an artificial biocatalytic cascade

Scheme 1

Design and modularization of a de novo designed biocatalytic cascade for production of chiral phenylglycinol from L-phenylalanine. Cell module 1 involves PAL catalyzed deamination of L-phenylalanine 1, Fdc1 and Pad1 mediates the conversion of trans-cinnamic acid 2 to styrene 3, SMO catalyzed oxidation of styrene 3 to styrene oxide 4, SpEH catalyzed hydrolysis of styrene oxide 4 to diol 5 and GoSCR catalyzed oxidation of diol 5 to 2-hydroxyacetophenone 6. Cell module 2 involves MVTA catalyzed asymmetric amination of 2-hydroxyacetophenone 6 to (S)-phenylglycinol 7. Cell module 3 involves BMTA catalyzed asymmetric amination of 2-hydroxyacetophenone 6 to (R)-phenylglycinol 7, as well as AlaDH catalyzed recycling of L-alanine and GDH catalyzed regeneration of NADH cofactor

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