Skip to main content

Table 1 Cascade biocatalysis for conversion of L-PA 1 to 2-HAP 6 and chiral phenylglycinol 7 with the mixture of lyophilized cell-free extracta

From: One-pot synthesis of (R)- and (S)-phenylglycinol from bio-based l-phenylalanine by an artificial biocatalytic cascade

Entry Sub. (mM) Time (h) Yield of 5 (%)d Yield of 6 (%)d Yield of 7 (%)d ee of 7 (%)e
1 10b 7  < 1 99
2 20b 12  < 1 99
3 10c 12 16.1  < 1 83.0  > 99 (R)
4 20c 24 32.5  < 1 66.8  > 99 (R)
5 10c 12 2.6 10.2 87.2  > 99 (S)
6 20c 24 4.3 25.3 70.4  > 99 (S)
  1. aThe reactions were conducted in 5 mL sodium phosphate buffer (100 mM, pH 7.5)
  2. b The reactions containing 10–20 mM L-PA, 0.5 mM NADH, 15 mg/mL PAL, 15 mg/mL Fdc1, 15 mg/mL Pad1, 20 mg/mL SMO, 10 mg/mL SpEH, 20 mg/mL GoSCR, at 30 °C and 200 rpm for 6 h
  3. c The reactions containing 10–20 mM L-PA, 0.5 mM NADH, 15 mg/mL PAL, 15 mg/mL Fdc1, 15 mg/mL Pad1, 20 mg/mL SMO, 10 mg/mL SpEH, 20 mg/mL GoSCR, 15 mg/mL MVTA or 15 mg/mL BMTA, 0.1 mM PLP, (R)-MBA (15–25 mM) or L-alanine (200 mM), at 30 °C and 200 rpm for 6 h
  4. d Yield was determined by GC
  5. e ee was determined by chiral GC