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Bioresources and Bioprocessing

Table 2 Preparation of (S)-7 and (R)-7 from L-PA 1 with the combined resting cell of E. coli (RFP-DEA-CGS) and E. coli (MVTA) or E. coli (EB-DGA) a

From: One-pot synthesis of (R)- and (S)-phenylglycinol from bio-based l-phenylalanine by an artificial biocatalytic cascade

Entry

L-PA 1 (mg)

Time (h)

7 (mg)

Yield of 7 (%)

ee of 7 (%)b

S.T.Y. (g/L d)

1

330.4

9

194.8

71.0

 > 99(S)

5.19

2

330.4

12

220.9

80.5

 > 99(R)

4.42

  1. S.T.Y.: space–time yield
  2. a The reactions were conducted in 100 mL sodium phosphate buffer (100 mM, pH 7.5). The reactions containing 2 mmol (330.4 mg) substrates, 20 g cdw/L E. coli (RFP-DEA-CGS) and 15 g cdw/L E. coli (MVTA) or 15 g cdw/L E. coli (RFP-DEA-CGS) and 20 g cdw/L E. coli (EB-DGA), 10–20 mM glucose, 0.1 mM PLP, 30 mM (R)-MBA or 400 mM L-Ala and 150 mM NH3/NH4Cl, at 25 °C
  3. b ee was determined by chiral GC
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