Entry
|
L-PA 1 (mg)
|
Time (h)
|
7 (mg)
|
Yield of 7 (%)
|
ee of 7 (%)b
|
S.T.Y. (g/L d)
|
---|
1
|
330.4
|
9
|
194.8
|
71.0
|
> 99(S)
|
5.19
|
2
|
330.4
|
12
|
220.9
|
80.5
|
> 99(R)
|
4.42
|
- S.T.Y.: space–time yield
- a The reactions were conducted in 100 mL sodium phosphate buffer (100 mM, pH 7.5). The reactions containing 2 mmol (330.4 mg) substrates, 20 g cdw/L E. coli (RFP-DEA-CGS) and 15 g cdw/L E. coli (MVTA) or 15 g cdw/L E. coli (RFP-DEA-CGS) and 20 g cdw/L E. coli (EB-DGA), 10–20 mM glucose, 0.1 mM PLP, 30 mM (R)-MBA or 400 mM L-Ala and 150 mM NH3/NH4Cl, at 25 °C
- b ee was determined by chiral GC