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Bioresources and Bioprocessing

Table 2 Preparation of (S)-7 and (R)-7 from L-PA 1 with the combined resting cell of E. coli (RFP-DEA-CGS) and E. coli (MVTA) or E. coli (EB-DGA) a

From: One-pot synthesis of (R)- and (S)-phenylglycinol from bio-based l-phenylalanine by an artificial biocatalytic cascade

Entry L-PA 1 (mg) Time (h) 7 (mg) Yield of 7 (%) ee of 7 (%)b S.T.Y. (g/L d)
1 330.4 9 194.8 71.0  > 99(S) 5.19
2 330.4 12 220.9 80.5  > 99(R) 4.42
  1. S.T.Y.: space–time yield
  2. a The reactions were conducted in 100 mL sodium phosphate buffer (100 mM, pH 7.5). The reactions containing 2 mmol (330.4 mg) substrates, 20 g cdw/L E. coli (RFP-DEA-CGS) and 15 g cdw/L E. coli (MVTA) or 15 g cdw/L E. coli (RFP-DEA-CGS) and 20 g cdw/L E. coli (EB-DGA), 10–20 mM glucose, 0.1 mM PLP, 30 mM (R)-MBA or 400 mM L-Ala and 150 mM NH3/NH4Cl, at 25 °C
  3. b ee was determined by chiral GC
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