Table 1 Enzymatic kinetics of WT glucanase and its disulfide bond mutants
From: Identification and structural analysis of a thermophilic β-1,3-glucanase from compost
Enzyme | Temperature (℃) | Vmax (μmol/min/mg) | Km (mg/mL) | kcat (s−1) | kcat/Km | ||
|---|---|---|---|---|---|---|---|
WT | 70 | 648.5 | 1.74 | 65.5 | 37.6 | ||
75 | 677.2 | 1.80 | 68.4 | 38.0 | |||
C160G | 70 | 717.0 | 1.81 | 72.4 | 40.0 | ||
75 | 730.0 | 2.01 | 73.7 | 36.7 | |||
C180I | 70 | 660.4 | 1.80 | 66.6 | 37.0 | ||
75 | 687.1 | 1.96 | 69.4 | 35.4 | |||