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Bioresources and Bioprocessing

Table 1 Optimization of HLADH–SBFC system
figure a

From: Biosynthesis of lactones from diols mediated by an artificial flavin

Entry

1,4-BD (mM)

HLADH (mg/mL)

NAD+ (mM)

SBFC (mM)

Time (h)

Conversionb (%)

Yieldc (%)

1a

20

0.3

0.5

0.1

6

100

> 99

2

20

0.3

0.1

0.1

6

100

> 99

3

20

0.3

0.05

0.1

12

100

> 99

4

20

0.3

0.1

0.05

6

100

> 99

5

20

0.3

0.1

0.01

12

60

60

  1. aReaction conditions: 1,4-BD (20 mM), NAD+ (0.5 mM), SBFC (0.1 mM), HLADH (0.3 g L−1), and catalase (20 U/mL) in 1 mL of aqueous Tris–HCl buffer (50mM, pH 8.0) at 30 °C
  2. bConversion was determined by GC analysis
  3. cYield was determined by GC analysis
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