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Table 1 Optimization of HLADH–SBFC system
figurea

From: Biosynthesis of lactones from diols mediated by an artificial flavin

Entry 1,4-BD (mM) HLADH (mg/mL) NAD+ (mM) SBFC (mM) Time (h) Conversionb (%) Yieldc (%)
1a 20 0.3 0.5 0.1 6 100 > 99
2 20 0.3 0.1 0.1 6 100 > 99
3 20 0.3 0.05 0.1 12 100 > 99
4 20 0.3 0.1 0.05 6 100 > 99
5 20 0.3 0.1 0.01 12 60 60
  1. aReaction conditions: 1,4-BD (20 mM), NAD+ (0.5 mM), SBFC (0.1 mM), HLADH (0.3 g L−1), and catalase (20 U/mL) in 1 mL of aqueous Tris–HCl buffer (50mM, pH 8.0) at 30 °C
  2. bConversion was determined by GC analysis
  3. cYield was determined by GC analysis