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Table 3 HLADH-catalyzed oxidative lactonization reaction mediated by different regeneration systems

From: Biosynthesis of lactones from diols mediated by an artificial flavin

Entry Regeneration system Yield (%) Time (h) TOFd (h−1)
1a SBFC, O2, catalase 43 48 2.23
2b Mb, H2O2 10 72 0.34
3c Laccase, ABTS, O2 38 72 1.32
  1. aFor SBFC regeneration method, SBFC (0.05 mM) and catalase (20 U/mL) were supplemented
  2. bFor Mb regeneration method, Mb (0.2 g L−1) and H2O2 (500 mM) were supplemented
  3. cThe data come from literature (Kara et al. 2013)
  4. dTOF value of catalytic system over the total reaction time. Standard reaction conditions: 1,4-BD (250 mM), NAD+ (0.1 mM), SBFC (0.05 mM), HLADH (0.3 g L−1), and catalase (20 U/mL) in 1 mL of aqueous Tris–HCl buffer (50mM, pH 8.0) at 30 °C