Biosynthesis of lactones from diols mediated by an artificial flavin

Bioresources and Bioprocessing

Table 3 HLADH-catalyzed oxidative lactonization reaction mediated by different regeneration systems

Entry	Regeneration system	Yield (%)	Time (h)	TOF^d (h⁻¹)
1^a	SBFC, O₂, catalase	43	48	2.23
2^b	Mb, H₂O₂	10	72	0.34
3^c	Laccase, ABTS, O₂	38	72	1.32

^aFor SBFC regeneration method, SBFC (0.05 mM) and catalase (20 U/mL) were supplemented
^bFor Mb regeneration method, Mb (0.2 g L⁻¹) and H₂O₂ (500 mM) were supplemented
^cThe data come from literature (Kara et al. 2013)
^dTOF value of catalytic system over the total reaction time. Standard reaction conditions: 1,4-BD (250 mM), NAD⁺ (0.1 mM), SBFC (0.05 mM), HLADH (0.3 g L⁻¹), and catalase (20 U/mL) in 1 mL of aqueous Tris–HCl buffer (50mM, pH 8.0) at 30 °C