Fig. 5From: Structure-guided protein engineering of ammonia lyase for efficient synthesis of sterically bulky unnatural amino acidsProgress curves of caffeic acid catalyzed by the purified enzymes of three EcMAL variants (M6, M7, M8). The enzymatic amination addition of caffeic acid (10 mM) was performed at 30 °C and pH 8.5 with a dose of each 0.5 mg/mL purified enzymes under the same conditionsBack to article page