Table 2 Confirmed mutations discovered in two E. coli LGE2-evolved strains adapted to inhibitors-containing LB media
Strain | Gene | Product description | Location | Class | Nucleotide | Codon | Protein change |
|---|---|---|---|---|---|---|---|
E. coli-L | rssB | Regulator of σS factor (RpoS) | Cytoplasm | Regulator | △T664 | Frame shifts | L245 ⟶ stop |
yqhA | UPF0114 protein | Plasma membrane | Regulator analog | G41T | TGG ⟶ TTG | W14L | |
IGR of yqhC/yqhD | 5’ untranslated region of yqhD | Cytoplasm | Regulator (promoter) | T77C | AAT ⟶ AAC | − 10 box change | |
E. coli-H | rssB | Regulator of σS factor (RpoS) | Cytoplasm | Regulator | △T664 | Frame shifts | L245 ⟶ stop |
yqhA | UPF0114 protein | Plasma membrane | Regulator analog | G41T | TGG ⟶ TTG | W14L | |
IGR of yqhC/yqhD | 5’ untranslated region of yqhD | Cytoplasm | Regulator (promoter) | T77C | AAT ⟶ AAC | − 10 box change | |
basR | Transcription regulator | Cytoplasm | Regulator | T292C | TAT ⟶ CAT | Y98H |