Fig. 5

Production of ATP under optimized conditions. A Production of ATP at different initial adenosine concentrations when only AtAdk and LhPPK were added to the reaction system. The orange, blue and black lines represent the reaction system of 10, 20 and 30 mM adenosine. B Production of ATP at different conditions with 30 mM adenosine as substrate. Black line: Only two enzymes of AtAdk and LhPPK were added to these system. Green line: Three enzymes of AtAdk, LhPPK and SlPPK were added simultaneously to the system. Red line: Two enzymes of AtAdk and LhPPK were added to the system at the beginning, and the SlPPK was added at 120 min. All measurements were carried out in triplicates