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Table 2 Kinetic parameters of the enzyme

From: In vitro biosynthesis of ATP from adenosine and polyphosphate

 

Substrate

Specific activity

Vmax

Km

kcat

kcat/Km

(U/mg)

(mM/min)

(mM)

(s−1)

 

LhPPK

AMP

172.3

0.86

5.70

92

16.1

 

ADP

19.1

    

AcPPK

AMP

41.5

    

RsPPK

AMP

1.7

    

SlPPK

ADP

282.9

1.4

0.61

140

229.5

AcsPPK

ADP

4.8

    

DaPPK

ADP

0.3