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Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Engineering Escherichia coli biofilm to increase contact surface for shikimate and L-malate production

Fig. 3

Engineering shikimate synthesis by biofilm self-assembly. A The schematic diagram of shikimate biosynthesis pathway in E. coli S1. B Comparing the color circle with starch in different E. coli. C The changes of color circle diameter, enzyme index, amylase concentration, and shikimate titer in different E. coli. D Conceptual graph of starch utilization with biofilm-based SpyTag/SpyCatcher covalently protein system for L-malate production. E Conceptual graph of two modes fermentation for shikimate production. F The schematic diagram of BLRS, containing the plasmid Ptac-EL222 and PJ-mKate. For C, n = 3. Error bars, mean ± s.d. Each gene encodes the following: Glu: glucose; G6P: glucose-6-phosphate; PYR: pyruvate; PEP: phosphoenolpyruvate; AcCoA: acetyl-CoA; E4P, erythrose-4-phosphate; DAHP, 3-deoxy-D-arabino-heptulosonate-7-phosphate; Ru5P, Ribulose 5-phosphate; DHQ, 3-dehydroquinic acid; DHS, 3-dehydroshikimate; SA, shikimate

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