Fig. 1From: A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coliScheme of plasmids construction, expression strains selection and protein secretion. The expression plasmid and accessory plasmid were co-transformed into BL21 (DE3) and selected by LB-Amp + Kan agar plate. Under the sequent induction of Arabinose and IPTG, the POI were secreted to supernatantBack to article page