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Bioresources and Bioprocessing
Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli

Fig. 4

Secretory expression and purification of the tags fusion protein. a Plasmid pET-Tag-THHly. b Secretory expression of the Tag-THHly fusion proteins. W whole-cell lysate, S supernatant. c Purification curve of STHHly. d, e Purification of STHHly (d) and TTHHly (e). S supernatant, L loading sample, FT flow through, E eluent. The arrows indicate Tag-THHly in b, STHHly in d, and TTHHly in e

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