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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli

Fig. 5

Secondary purification of the tags and verification of the immunogenicity. a Technical map of the purification procedure. b Digestion of STHHly and TTHHly with TEV enzyme. c Purification of S tag and T7 tag. L loading sample, FT flow through, E eluent. The samples were detected by a mouse anti-His tag antibody. d, e Verification of the immunogenicity of the tags in FT (d), eluent and supernatant (e). Elu eluent

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