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Bioresources and Bioprocessing
Fig. 7 | Bioresources and Bioprocessing

Fig. 7

From: A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli

Fig. 7

Determination of the anti-microbial/anti-tumor activity of rCeA/rMel. a, b Comparison of the anti-microbial activity of rCeA/cCeA (a) and anti-tumor activity of rMel/cMel (b). c, d Comparison of the tumor lysis capacity (c) and pro-apoptosis effects of rMel and cMel (d). E. coli 25,922 cells were incubated with different concentrations of cCeA and rCeA for 18 h, then the OD600 values were measured. A549 cells were incubated with different concentrations of cMel and rMel for 8 h, the numbers of living cells were detected with CCK8 reagent. For apoptosis detection, A549 cells were incubated with 10 μM melittin for 1 h, and subsequently labeled with Annexin V-FITC/PI and analyzed with a CytoFlex S

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