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Table 1 Summary of the purification of Cecropin A

From: A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli

Purification step Concentration (μg/mL) Volume (mL) Content (μg) Recovery (%) Overall recover (%)
Cell free supernatant 4.37 100 436.86 100 100
Nickel affinity chromatography 118.61 3.4 403.27 92.31 92.31
Sample for Ultrafiltration 118.61 1.5 177.92
Ultrafiltration 1603.7 0.1 160.37 90.14 83.21
Sample for cleavage 1603.7 0.08 128.29
CeA in the sample 260.06 0.08 20.8
Ultrafiltration 867.91 0.02 17.36 83.46 69.45