Table 1 Summary of the purification of Cecropin A
Purification step | Concentration (μg/mL) | Volume (mL) | Content (μg) | Recovery (%) | Overall recover (%) |
|---|---|---|---|---|---|
Cell free supernatant | 4.37 | 100 | 436.86 | 100 | 100 |
Nickel affinity chromatography | 118.61 | 3.4 | 403.27 | 92.31 | 92.31 |
Sample for Ultrafiltration | 118.61 | 1.5 | 177.92 | – | – |
Ultrafiltration | 1603.7 | 0.1 | 160.37 | 90.14 | 83.21 |
Sample for cleavage | 1603.7 | 0.08 | 128.29 | – | – |
CeA in the sample | 260.06 | 0.08 | 20.8 | – | – |
Ultrafiltration | 867.91 | 0.02 | 17.36 | 83.46 | 69.45 |