Table 2 Summary of the purification of Melittin
Purification step | Concentration (μg/mL) | Volume (mL) | Content (μg) | Recovery (%) | Overall recovery (%) |
|---|---|---|---|---|---|
Cell free supernatant | 16.39 | 100 | 1639.41 | 100 | 100 |
Nickel affinity chromatography | 347.68 | 4.2 | 1455.63 | 88.79 | 88.79 |
Sample for ultrafiltration | 347.68 | 2 | 695.36 | – | – |
Ultrafiltration | 5984.22 | 0.1 | 598.42 | 86.06 | 76.41 |
Sample for cleavage | 5984.22 | 0.08 | 478.74 | – | – |
Mel in the sample | 755.9 | 0.08 | 60.47 | – | – |
Ultrafiltration | 2091.5 | 0.02 | 41.83 | 69.17 | 52.85 |