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Table 2 Summary of the purification of Melittin

From: A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli

Purification step Concentration (μg/mL) Volume (mL) Content (μg) Recovery (%) Overall recovery (%)
Cell free supernatant 16.39 100 1639.41 100 100
Nickel affinity chromatography 347.68 4.2 1455.63 88.79 88.79
Sample for ultrafiltration 347.68 2 695.36
Ultrafiltration 5984.22 0.1 598.42 86.06 76.41
Sample for cleavage 5984.22 0.08 478.74
Mel in the sample 755.9 0.08 60.47
Ultrafiltration 2091.5 0.02 41.83 69.17 52.85