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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Characterization of Argonaute nucleases from mesophilic bacteria Paenibacillus borealis and Brevibacillus laterosporus

Fig. 2

Enzymatic characterization of PbAgo and BlAgo in vitro guided by the 5′-P ssDNA. a Effect of temperature on PbAgo and BlAgo activity mediated by the 5′-P gDNA. b Effects of different divalent cations on PbAgo and BlAgo activity mediated by the 5′-P gDNA. c Effects of Mn2+ concentrations on PbAgo and BlAgo activity mediated by the 5′-P gDNA. d Kinetic performance of PbAgo and BlAgo mediated by 5′-P and 5′-OH gDNAs. Error bars represent the SDs of three independent experiments

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