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Bioresources and Bioprocessing
Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: Characterization of Argonaute nucleases from mesophilic bacteria Paenibacillus borealis and Brevibacillus laterosporus

Fig. 4

Cleavage of pUC19 by PbAgo and BlAgo. a PbAgo cleaves pUC19 at 37 °C guided by a pair of 5′-P gDNAs. BlAgo cleaves pUC19 at 65 °C guided by a pair of 5′-P gDNAs. n Kinetic performance of PbAgo- and BlAgo-cleaving pUC19. c Effect of GC content of 50 bp plasmid target fragment on PbAgo and BlAgo cleavage activity. FW/RV gDNA, forward and reverse gDNAs; M, molecular weight marker; OC, open circular plasmid; Lin, linearized plasmid; SC, supercoiled plasmid

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