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Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Characterization of Argonaute nucleases from mesophilic bacteria Paenibacillus borealis and Brevibacillus laterosporus

Fig. 5

Small molecule detection by PbAgo and BlAgo and allosteric transcription factors. a Schematic diagram of small molecule detection by PbAgo and BlAgo and allosteric transcription factors. b EMSA with HosA and irregular dsDNA containing the HosA-binding motif. c EMSA evaluation of HosA allosteric activity in the presence of p-HBA. d The denaturing polyacrylamide gel electrophoresis (PAGE) result of PbAgo detecting p-HBA. The denaturing PAGE with irregular dsDNA (lane 1), with irregular dsDNA incubated with HosA (lane 2), with irregular dsDNA incubated with HosA and p-HBA (lane 3), with irregular dsDNA incubated with HosA and PbAgo/gDNA (lane 4), and with irregular dsDNA incubated with HosA, p-HBA, and PbAgo/gDNA (lane 5). e The denaturing PAGE result of BlAgo detected p-HBA. The denaturing PAGE with irregular dsDNA (lane 1), with irregular dsDNA incubated with HosA (lane 2), with irregular dsDNA incubated with HosA and p-HBA (lane 3), with irregular dsDNA incubated with HosA and BlAgo/gDNA (lane 4), and with irregular dsDNA incubated with HosA, p-HBA, and BlAgo/gDNA (lane 5)

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