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Bioresources and Bioprocessing
Fig. 6 | Bioresources and Bioprocessing

Fig. 6

From: Strain engineering and bioprocessing strategies for biobased production of porphobilinogen in Escherichia coli

Fig. 6

Bioreactor cultivation of DSL∆iclR∆sdhA and DSL-D9∆iclR∆sdhA for PBG biosynthesis under aerobic conditions. Time profiles of cell density (OD600), glycerol consumption and metabolite production profiles, acetate and PBG percentage yields, and extracellular accumulation of porphyrins (represented by the absorbance readings of the Soret peak (A405) and Q-band (A495)) are shown. The percentage yields of acetate/PBG and absorbance readings of porphyrin compounds are calculated/measured based on the consumed glycerol at end of cultivation. Final PBG titer comparison for DSL, DSL∆iclR∆sdhA, and DSL-D9∆iclR∆sdhA was deemed to be statistically significant (Additional file 1: Table S4). (I) DSL∆iclR∆sdhA, (II) DSL-D9∆iclR∆sdhA. All values are reported as means ± SD (n = 2)

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