Table 1 Strains and plasmids used in this study
Strains or plasmids | Description | References |
|---|---|---|
Plasmids | ||
pTrc99a | High-copy expression plasmid, AmpR | Lab collection |
pTrc99a-J23106-doy-phaP-pntAB | pTrc99a containing dhaBCE and gdrAB genes from K. pneumoniae under the control of J23106 promoter, an artificial operon containing yqhD gene from E. coli, phaP gene from Azotobacter sp., and pntAB genes from E. coli under the control of J23100 promoter | Zhang et al. (2021) |
pTrc99a-J23106-doy-phaP-pntAB-glpD1 | pTrc99a-J23106-doy-phaP-pntAB containing glpD gene under the control of V. natriegens’ dnaG promoter | This study |
pTrc99a-J23106-doy-phaP-pntAB-glpD2 | pTrc99a-J23106-doy-phaP-pntAB containing glpD gene under the control of V. natriegens’ patZ promoter | This study |
pTrc99a-J23106-doy-phaP-pntAB-glpD3 | pTrc99a-J23106-doy-phaP-pntAB containing glpD gene under the control of V. natriegens’ ribC promoter | This study |
pTrc99a-dhaBCE-aldH | pTrc99a containing dhaBCE and gdrAB genes from K. pneumoniae under the control of J23106 promoter, an artificial operon containing aldH gene from E. coli and phaP gene from Azotobacter sp. under the control of J23100 promoter | This study |
pTrc99a-dhaBCE-aldH-glpD3 | pTrc99a-dhaBCE-aldH containing glpD gene under the control of ribC promoter | This study |
Strains | ||
V. natriegens ATCC14048 | Wild-type V. natriegens | Lab collection |
VN09 | V. natriegens ATCC14048, ΔadhEΔldhAΔpflΔpta-ackAΔarcAΔglpRΔsthA ΔfrdABCDΔaldAΔaldB | Zhang et al. (2021) |
VN10 | VN9, harboring pTrc99a-J23106-doy-phaP-pntAB | Zhang et al. (2021) |
VN10ΔglpD | VN10, ΔglpD | This study |
VN11 | VN09, ΔglpD, harboring pTrc99a-J23106-doy-phaP-pntAB-glpD1 | This study |
VN12 | VN09, ΔglpD, harboring pTrc99a-J23106-doy-phaP-pntAB-glpD2 | This study |
VN13 | VN09, ΔglpD, harboring pTrc99a-J23106-doy-phaP-pntAB-glpD3 | This study |
VN05 | V. natriegens ATCC14048, ΔadhEΔldhAΔpflΔpta-ackAΔfrdABCD | This study |
VN14 | VN05, harboring pTrc99a-dhaBCE-aldH | This study |
VN05ΔglpD | VN05, ΔglpD, | This study |
VN15 | VN05ΔglpD, pTrc99a-dhaBCE-aldH-glpD3 | This study |