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Table 1 Strains and plasmids used in this study

From: Development of a plasmid stabilization system in Vibrio natriegens for the high production of 1,3-propanediol and 3-hydroxypropionate

Strains or plasmids Description References
Plasmids   
 pTrc99a High-copy expression plasmid, AmpR Lab collection
 pTrc99a-J23106-doy-phaP-pntAB pTrc99a containing dhaBCE and gdrAB genes from K. pneumoniae under the control of J23106 promoter, an artificial operon containing yqhD gene from E. coli, phaP gene from Azotobacter sp., and pntAB genes from E. coli under the control of J23100 promoter Zhang et al. (2021)
 pTrc99a-J23106-doy-phaP-pntAB-glpD1 pTrc99a-J23106-doy-phaP-pntAB containing glpD gene under the control of V. natriegensdnaG promoter This study
 pTrc99a-J23106-doy-phaP-pntAB-glpD2 pTrc99a-J23106-doy-phaP-pntAB containing glpD gene under the control of V. natriegenspatZ promoter This study
 pTrc99a-J23106-doy-phaP-pntAB-glpD3 pTrc99a-J23106-doy-phaP-pntAB containing glpD gene under the control of V. natriegensribC promoter This study
 pTrc99a-dhaBCE-aldH pTrc99a containing dhaBCE and gdrAB genes from K. pneumoniae under the control of J23106 promoter, an artificial operon containing aldH gene from E. coli and phaP gene from Azotobacter sp. under the control of J23100 promoter This study
 pTrc99a-dhaBCE-aldH-glpD3 pTrc99a-dhaBCE-aldH containing glpD gene under the control of ribC promoter This study
Strains   
 V. natriegens ATCC14048 Wild-type V. natriegens Lab collection
 VN09 V. natriegens ATCC14048, ΔadhEΔldhAΔpflΔpta-ackAΔarcAΔglpRΔsthA ΔfrdABCDΔaldAΔaldB Zhang et al. (2021)
 VN10 VN9, harboring pTrc99a-J23106-doy-phaP-pntAB Zhang et al. (2021)
 VN10ΔglpD VN10, ΔglpD This study
 VN11 VN09, ΔglpD, harboring pTrc99a-J23106-doy-phaP-pntAB-glpD1 This study
 VN12 VN09, ΔglpD, harboring pTrc99a-J23106-doy-phaP-pntAB-glpD2 This study
 VN13 VN09, ΔglpD, harboring pTrc99a-J23106-doy-phaP-pntAB-glpD3 This study
 VN05 V. natriegens ATCC14048, ΔadhEΔldhAΔpflΔpta-ackAΔfrdABCD This study
 VN14 VN05, harboring pTrc99a-dhaBCE-aldH This study
 VN05ΔglpD VN05, ΔglpD, This study
 VN15 VN05ΔglpD, pTrc99a-dhaBCE-aldH-glpD3 This study