Table 1 Effect of PEG–potassium phosphate systems variables on the recovery of MPs and PLA₂

S1

400

0.33

15

181.90 ± 23.31^{b,c,d,e,f,g,h,i,j}

13.72 ± 3.47^n,o

92.80 ± 25.87^a

3.76 ± 1.55 ^{g,h,i,j,k,l,m}

0.48 ± 0.10^e

S2

25

178.95 ± 15.41^{b,c,d,e,f,g,h,i,j}

15.59 ± 0.31^n,o

66.58 ± 12.37^a,b,c,d,e

5.61 ± 0.98^{e,f,g,h,i,j,k,l}

1.01 ± 0.19^e

S3

35

161.32 ± 16.78^{d,e,f,g,h,i,j,k}

15.72 ± 2.29^n,o

72.14 ± 5.09^a,b,c

5.58 ± 0.31^{e,f,g,h,i,j,k,l}

1.10 ± 0.20^e

S4

45

196.23 ± 0.85^{b,c,d,e,f,g,h,i}

16.46 ± 3.37 ^m,n,o

63.70 ± 3.18^{a,b,c,d,e,f,g}

7.74 ± 0.40^c,d,e,f

13.20 ± 3.13^d,e

S5

1.00

15

144.81 ± 27.25^{e,f,g,h,i,j,k,l}

25.07 ± 2.62 ^l,m,n,o

88.75 ± 32.48^a

1.71 ± 0.65 ^m,n

5.80 ± 1.46^e

S6

25

131.60 ± 34.51^{e,f,g,h,i,j,k,l}

13.47 ± 8.50^n,o

47.93 ± 2.14^{b,c,d,e,f,g,h,i,j}

2.33 ± 0.65^j,k,l,m,n

5.11 ± 2.75^e

S7

1

112.85 ± 68.77 ^h,i,j,k,l

79.20 ± 3.12 ^k,l,m,n,o

47.65 ± 3.07^{b,c,d,e,f,g,h,i,j}

2.14 ± 1.38 ^l,m,n

31.38 ± 1.75^d,e

S8

45

95.52 ± 57.87^i,j,k,l

115.37 ± 22.92^{i,j,k,l,m,n,o}

4.29 ± 3.48 ^m,n

2.58 ± 2.58^i,j,k,l,m,n

479.90 ± 346.20^a,b

S9

3.00

15

158.67 ± 10.37^{d,e,f,g,h,i,j,k}

89.18 ± 4.93^j,k,l,m,n,o

54.90 ± 2.20^{b,c,d,e,f,g,h}

2.24 ± 0.22 ^k,l,m,n

25.08 ± 1.69^d,e

S10

25

137.03 ± 21.98^{e,f,g,h,i,j,k,l}

173.74 ± 11.75 ^h,i,j,k

47.03 ± 6.38^{c,d,e,f,g,h,i,j}

2.07 ± 0.09 ^l,m,n

54.98 ± 8.48^d,e

S11

35

155.22 ± 1.99^{d,e,f,g,h,i,j,k,l}

336.73 ± 10.54^d,e

39.83 ± 3.85^{d,e,f,g,h,i,j,k,l}

3.01 ± 0.27 ^{h,i,j,k,l,m,n}

131.22 ± 14.59^c,d,e

S12

45

161.32 ± 8.99^{d,e,f,g,h,i,j,k}

474.34 ± 24.89^a,b,c

41.94 ± 2.23^{d,e,f,g,h,i,j,k,l}

2.89 ± 0.12^i,j,k,l,m,n

171.12 ± 14.66^c,d,e

S13

1000

0.33

15

177.77 ± 30.23^{b,c,d,e,f,g,h,i,j}

125.35 ± 25.28^i,j,k,l,m,n

48.88 ± 4.61^{b,c,d,e,f,g,h,i}

7.24 ± 1.05^c,d,e,f,g

101.62 ± 17.47^d,e

S14

25

232.02 ± 21.22^a,b,c,d,e,f

151.23 ± 33.00^i,j,k

77.34 ± 5.71^a,b

5.98 ± 0.11^e,f,g,h,i

77.12 ± 14.50^d,e

S15

35

275.38 ± 13.85^a,b

205.21 ± 8.08^f,g,h,i,j

67.91 ± 9.82^a,b,c,d

8.94 ± 0.83^b,c,d,e

135.50 ± 20.82^c,d,e

S16

45

269.58 ± 7.49^a,b,c

167.63 ± 11.26 ^h,i,j,k

65.02 ± 2.97^a,b,c,d,e,f

10.40 ± 0.48^a,b,c

128.85 ± 5.83^c,d,e

S17

1.00

15

186.26 ± 34.06^{b,c,d,e,f,g,h,i,j}

184.67 ± 5.86 ^g,h,i,j,k

47.20 ± 4.26^{c,d,e,f,g,h,i,j}

4.31 ± 0.44^{f,g,h,i,j,k,l,m}

88.50 ± 9.09^d,e

S18

25

253.13 ± 49.13^a,b,c,d

276.71 ± 28.48^e,f,g,h

42.23 ± 6.61^{c,d,e,f,g,h,i,j,k,l}

6.57 ± 0.35^d,e,f,g

148.33 ± 9.33^c,d,e

S19

35

234.02 ± 29.48^a,b,c,d,e

299.16 ± 31.49^e,f,g

45.18 ± 3.42^{c,d,e,f,g,h,i,j,k}

5.73 ± 0.43^{e,f,g,h,i,j,k}

147.51 ± 11.76^c,d,e

S20

45

147.58 ± 35.78^{e,f,g,h,i,j,k,l}

211.41 ± 72.20^f,g,h,i

37.25 ± 8.58^{e,f,g,h,i,j,k,l}

4.72 ± 0.12^{f,g,h,i,j,k,l,m}

127.09 ± 21.61^c,d,e

S21

3.00

15

227.59 ± 21.16^{a,b,c,d,e,f,g}

422.05 ± 23.12^b,c,d

29.12 ± 1.56 ^{h,i,j,k,l,m,n}

4.78 ± 0.32^{f,g,h,i,j,k,l,m}

177.77 ± 7.00^c,d,e

S22

25

229.25 ± 20.91^{a,b,c,d,e,f,g}

319.55 ± 11.02^d,e,f

29.50 ± 0.99 ^{h,i,j,k,l,m,n}

6.45 ± 0.39^d,e,f,g,h

180.56 ± 0.99^c,d,e

S23

35

331.84 ± 23.41^a

282.01 ± 22.95^e,f,g,h

30.32 ± 1.91 ^h,i,j,k,l,m

7.83 ± 0.46^b,c,d,e,f

132.76 ± 5.44^c,d,e

S24

45

232.78 ± 28.75^a,b,c,d,e

207.86 ± 13.12^f,g,h,i

17.94 ± 0.17^j,k,l,m,n

8.82 ± 0.97^b,c,d,e

157.76 ± 6.55^c,d,e

S25

3350

0.33

15

84.14 ± 12.82^j,k,l

76.58 ± 15.32 ^k,l,m,n,o

40.39 ± 2.61^{d,e,f,g,h,i,j,k,l}

4.42 ± 0.24^{f,g,h,i,j,k,l,m}

84.59 ± 21.20^d,e

S26

25

71.20 ± 5.95 ^k,l

141.25 ± 1.62^i,j,k,l

35.07 ± 2.35^{f,g,h,i,j,k,l}

3.93 ± 0.07 ^{g,h,i,j,k,l,m}

157.91 ± 13.06^c,d,e

S27

35

50.09 ± 18.70 ^l

117.09 ± 3.74^{i,j,k,l,m,n,o}

46.37 ± 2.36^{c,d,e,f,g,h,i,j}

2.76 ± 1.02^i,j,k,l,m,n

132.69 ± 7.86^c,d,e

S28

45

126.89 ± 16.39^{f,g,h,i,j,k,l}

147.43 ± 4.98^i,j,k

48.92 ± 1.41^{b,c,d,e,f,g,h,i}

6.46 ± 0.74^d,e,f,g,h

150.94 ± 6.52^c,d,e

S29

1.00

15

125.59 ± 11.37 ^g,h,i,j,k,l

84.81 ± 3.30 ^k,l,m,n,o

34.05 ± 3.74 ^{g,h,i,j,k,l,m}

3.71 ± 0.17 ^{g,h,i,j,k,l,m}

51.28 ± 6.86^d,e

S30

25

142.22 ± 14.86^{e,f,g,h,i,j,k,l}

134.14 ± 4.89^i,j,k,l,m

27.05 ± 0.63 ^{h,i,j,k,l,m,n}

5.83 ± 0.54^e,f,g,h,i,j

110.18 ± 2.63^d,e

S31

35

169.90 ± 32.55^{c,d,e,f,g,h,i,j,k}

119.49 ± 7.61^i,j,k,l,m,n

25.28 ± 2.63 ^{h,i,j,k,l,m,n}

8.71 ± 1.60^b,c,d,e

121.80 ± 4.67^d,e

S32

45

228.18 ± 58.38^{a,b,c,d,e,f,g}

168.38 ± 14.32 ^h,i,j,k

18.64 ± 3.96^j,k,l,m,n

13.69 ± 2.35^a

212.18 ± 30.36^b,c

S33

3.00

15

ND

ND

ND

ND

ND

S34

25

202.36 ± 19.32^{b,c,d,e,f,g,h}

546.37 ± 6.58^a

21.63 ± 1.89^i,j,k,l,m,n

5.97 ± 0.17^e,f,g,h,i

326.12 ± 18.81^b,c

S35

35

228.30 ± 25.38^{a,b,c,d,e,f,g}

538.82 ± 19.48^a,b

15.15 ± 1.47 ^k,l,m,n

9.73 ± 1.78^b,c,d

450.75 ± 34.06^b

S36

45

211.20 ± 23.60^{b,c,d,e,f,g,h}

373.49 ± 154.91^c,d,e

14.24 ± 2.94 ^l,m,n

11.36 ± 1.77^a,b

676.37 ± 104.65^a