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Table 1 Effect of PEG–potassium phosphate systems variables on the recovery of MPs and PLA2

From: Development of aqueous two-phase systems-based approaches for the selective recovery of metalloproteases and phospholipases A2 toxins from Crotalus molossus nigrescens venom

System identifier System parameters Top phase recovery percentage (%R) Top phase purification factor (PF)
PEG molecular weight (g mol−1) VR TLL (% w/w) MPs PLA2 Total protein MPs PLA2
S1 400 0.33 15 181.90 ± 23.31b,c,d,e,f,g,h,i,j 13.72 ± 3.47n,o 92.80 ± 25.87a 3.76 ± 1.55 g,h,i,j,k,l,m 0.48 ± 0.10e
S2 25 178.95 ± 15.41b,c,d,e,f,g,h,i,j 15.59 ± 0.31n,o 66.58 ± 12.37a,b,c,d,e 5.61 ± 0.98e,f,g,h,i,j,k,l 1.01 ± 0.19e
S3 35 161.32 ± 16.78d,e,f,g,h,i,j,k 15.72 ± 2.29n,o 72.14 ± 5.09a,b,c 5.58 ± 0.31e,f,g,h,i,j,k,l 1.10 ± 0.20e
S4 45 196.23 ± 0.85b,c,d,e,f,g,h,i 16.46 ± 3.37 m,n,o 63.70 ± 3.18a,b,c,d,e,f,g 7.74 ± 0.40c,d,e,f 13.20 ± 3.13d,e
S5 1.00 15 144.81 ± 27.25e,f,g,h,i,j,k,l 25.07 ± 2.62 l,m,n,o 88.75 ± 32.48a 1.71 ± 0.65 m,n 5.80 ± 1.46e
S6 25 131.60 ± 34.51e,f,g,h,i,j,k,l 13.47 ± 8.50n,o 47.93 ± 2.14b,c,d,e,f,g,h,i,j 2.33 ± 0.65j,k,l,m,n 5.11 ± 2.75e
S7 1 112.85 ± 68.77 h,i,j,k,l 79.20 ± 3.12 k,l,m,n,o 47.65 ± 3.07b,c,d,e,f,g,h,i,j 2.14 ± 1.38 l,m,n 31.38 ± 1.75d,e
S8 45 95.52 ± 57.87i,j,k,l 115.37 ± 22.92i,j,k,l,m,n,o 4.29 ± 3.48 m,n 2.58 ± 2.58i,j,k,l,m,n 479.90 ± 346.20a,b
S9 3.00 15 158.67 ± 10.37d,e,f,g,h,i,j,k 89.18 ± 4.93j,k,l,m,n,o 54.90 ± 2.20b,c,d,e,f,g,h 2.24 ± 0.22 k,l,m,n 25.08 ± 1.69d,e
S10 25 137.03 ± 21.98e,f,g,h,i,j,k,l 173.74 ± 11.75 h,i,j,k 47.03 ± 6.38c,d,e,f,g,h,i,j 2.07 ± 0.09 l,m,n 54.98 ± 8.48d,e
S11 35 155.22 ± 1.99d,e,f,g,h,i,j,k,l 336.73 ± 10.54d,e 39.83 ± 3.85d,e,f,g,h,i,j,k,l 3.01 ± 0.27 h,i,j,k,l,m,n 131.22 ± 14.59c,d,e
S12 45 161.32 ± 8.99d,e,f,g,h,i,j,k 474.34 ± 24.89a,b,c 41.94 ± 2.23d,e,f,g,h,i,j,k,l 2.89 ± 0.12i,j,k,l,m,n 171.12 ± 14.66c,d,e
S13 1000 0.33 15 177.77 ± 30.23b,c,d,e,f,g,h,i,j 125.35 ± 25.28i,j,k,l,m,n 48.88 ± 4.61b,c,d,e,f,g,h,i 7.24 ± 1.05c,d,e,f,g 101.62 ± 17.47d,e
S14 25 232.02 ± 21.22a,b,c,d,e,f 151.23 ± 33.00i,j,k 77.34 ± 5.71a,b 5.98 ± 0.11e,f,g,h,i 77.12 ± 14.50d,e
S15 35 275.38 ± 13.85a,b 205.21 ± 8.08f,g,h,i,j 67.91 ± 9.82a,b,c,d 8.94 ± 0.83b,c,d,e 135.50 ± 20.82c,d,e
S16 45 269.58 ± 7.49a,b,c 167.63 ± 11.26 h,i,j,k 65.02 ± 2.97a,b,c,d,e,f 10.40 ± 0.48a,b,c 128.85 ± 5.83c,d,e
S17 1.00 15 186.26 ± 34.06b,c,d,e,f,g,h,i,j 184.67 ± 5.86 g,h,i,j,k 47.20 ± 4.26c,d,e,f,g,h,i,j 4.31 ± 0.44f,g,h,i,j,k,l,m 88.50 ± 9.09d,e
S18 25 253.13 ± 49.13a,b,c,d 276.71 ± 28.48e,f,g,h 42.23 ± 6.61c,d,e,f,g,h,i,j,k,l 6.57 ± 0.35d,e,f,g 148.33 ± 9.33c,d,e
S19 35 234.02 ± 29.48a,b,c,d,e 299.16 ± 31.49e,f,g 45.18 ± 3.42c,d,e,f,g,h,i,j,k 5.73 ± 0.43e,f,g,h,i,j,k 147.51 ± 11.76c,d,e
S20 45 147.58 ± 35.78e,f,g,h,i,j,k,l 211.41 ± 72.20f,g,h,i 37.25 ± 8.58e,f,g,h,i,j,k,l 4.72 ± 0.12f,g,h,i,j,k,l,m 127.09 ± 21.61c,d,e
S21 3.00 15 227.59 ± 21.16a,b,c,d,e,f,g 422.05 ± 23.12b,c,d 29.12 ± 1.56 h,i,j,k,l,m,n 4.78 ± 0.32f,g,h,i,j,k,l,m 177.77 ± 7.00c,d,e
S22 25 229.25 ± 20.91a,b,c,d,e,f,g 319.55 ± 11.02d,e,f 29.50 ± 0.99 h,i,j,k,l,m,n 6.45 ± 0.39d,e,f,g,h 180.56 ± 0.99c,d,e
S23 35 331.84 ± 23.41a 282.01 ± 22.95e,f,g,h 30.32 ± 1.91 h,i,j,k,l,m 7.83 ± 0.46b,c,d,e,f 132.76 ± 5.44c,d,e
S24 45 232.78 ± 28.75a,b,c,d,e 207.86 ± 13.12f,g,h,i 17.94 ± 0.17j,k,l,m,n 8.82 ± 0.97b,c,d,e 157.76 ± 6.55c,d,e
S25 3350 0.33 15 84.14 ± 12.82j,k,l 76.58 ± 15.32 k,l,m,n,o 40.39 ± 2.61d,e,f,g,h,i,j,k,l 4.42 ± 0.24f,g,h,i,j,k,l,m 84.59 ± 21.20d,e
S26 25 71.20 ± 5.95 k,l 141.25 ± 1.62i,j,k,l 35.07 ± 2.35f,g,h,i,j,k,l 3.93 ± 0.07 g,h,i,j,k,l,m 157.91 ± 13.06c,d,e
S27 35 50.09 ± 18.70 l 117.09 ± 3.74i,j,k,l,m,n,o 46.37 ± 2.36c,d,e,f,g,h,i,j 2.76 ± 1.02i,j,k,l,m,n 132.69 ± 7.86c,d,e
S28 45 126.89 ± 16.39f,g,h,i,j,k,l 147.43 ± 4.98i,j,k 48.92 ± 1.41b,c,d,e,f,g,h,i 6.46 ± 0.74d,e,f,g,h 150.94 ± 6.52c,d,e
S29 1.00 15 125.59 ± 11.37 g,h,i,j,k,l 84.81 ± 3.30 k,l,m,n,o 34.05 ± 3.74 g,h,i,j,k,l,m 3.71 ± 0.17 g,h,i,j,k,l,m 51.28 ± 6.86d,e
S30 25 142.22 ± 14.86e,f,g,h,i,j,k,l 134.14 ± 4.89i,j,k,l,m 27.05 ± 0.63 h,i,j,k,l,m,n 5.83 ± 0.54e,f,g,h,i,j 110.18 ± 2.63d,e
S31 35 169.90 ± 32.55c,d,e,f,g,h,i,j,k 119.49 ± 7.61i,j,k,l,m,n 25.28 ± 2.63 h,i,j,k,l,m,n 8.71 ± 1.60b,c,d,e 121.80 ± 4.67d,e
S32 45 228.18 ± 58.38a,b,c,d,e,f,g 168.38 ± 14.32 h,i,j,k 18.64 ± 3.96j,k,l,m,n 13.69 ± 2.35a 212.18 ± 30.36b,c
S33 3.00 15 ND ND ND ND ND
S34 25 202.36 ± 19.32b,c,d,e,f,g,h 546.37 ± 6.58a 21.63 ± 1.89i,j,k,l,m,n 5.97 ± 0.17e,f,g,h,i 326.12 ± 18.81b,c
S35 35 228.30 ± 25.38a,b,c,d,e,f,g 538.82 ± 19.48a,b 15.15 ± 1.47 k,l,m,n 9.73 ± 1.78b,c,d 450.75 ± 34.06b
S36 45 211.20 ± 23.60b,c,d,e,f,g,h 373.49 ± 154.91c,d,e 14.24 ± 2.94 l,m,n 11.36 ± 1.77a,b 676.37 ± 104.65a
  1. When the enzymatic activity was too low to be quantified, non-detected (ND) is indicated. Values in the same column with different letters are significantly different (p < 0.05)