Development of aqueous two-phase systems-based approaches for the selective recovery of metalloproteases and phospholipases A2 toxins from Crotalus molossus nigrescens venom

Bioresources and Bioprocessing

Table 2 NaCl concentration effect on the recovery of MPs and PLA₂ in PEG–potassium phosphate systems

System identifier	System parameters			Top phase recovery percentage (%R)			Top phase purification factor (PF)
System identifier	V_R	TLL (% w/w)	NaCl (%w/w)	MPs	PLA₂	Total protein	MPs	PLA₂
S37	0.33	15	1	53.83 ± 16.68^a,b	180.54 ± 6.23^a	50.37 ± 2.76^a	2.14 ± 0.67^a,b	143.72 ± 3.12^a,b
S38			3	53.19 ± 15.25^a,b	141.25 ± 5.64^a,b	40.17 ± 2.11^b	2.70 ± 0.85^a,b	141.64 ± 10.62^a,b,c
S39			5	46.25 ± 9.87^a,b,c,d	55.78 ± 2.74^b	30.60 ± 4.16^c,d	2.65 ± 1.07^a,b	59.43 ± 20.84^e,f
S40		25	1	45.40 ± 6.39^a,b,c,d	126.91 ± 1.56^a,b	43.26 ± 2.94^b	2.09 ± 0.23^a,b	118.66 ± 9.52^a,b,c,d,e
S41			3	51.24 ± 9.42^a,b,c	141.88 ± 3.07^a,b	40.42 ± 0.66^b	2.54 ± 0.49^a,b	140.51 ± 4.11^a,b,c,d
S42			5	56.43 ± 1.95^a,b	66.73 ± 4.40^b	33.12 ± 1.74^c	3.41 ± 3.41^a,b	81.40 ± 8.71^d,e
S43	1.00	15	1	ND	113.16 ± 56.77^a,b	27.81 ± 1.23^c,d,e	ND	85.00 ± 42.67^b,c,d,e
S44			3	12.52 ± 7.96^c,d,e	106.64 ± 53.36^a,b	25.32 ± 1.10^d,e,f	0.52 ± 0.33^a,b	143.27 ± 13.39^a,b
S45			5	66.55 ± 10.70^a	121.23 ± 3.51^a,b	19.58 ± 0.41 ^f,g	3.79 ± 0.66^a	137.83 ± 6.12^a,b,c,d
S46		25	1	18.84 ± 131.67^b,c,d,e	131.67 ± 16.14^a,b	23.51 ± 1.06^e,f,g	0.81 ± 0.64^a,b	0.67 ± 0.00 ^f
S47			3	9.73 ± 5.94^d,e	100.81 ± 51.67^a,b	24.58 ± 0.51^d,e,f	0.42 ± 0.26^a,b	83.39 ± 42.06^c,d,e
S48			5	26.85 ± 20.26^a,b,c,d,e	123.20 ± 6.50^a,b	17.19 ± 1.04 h	2.30 ± 1.33^a,b	154.71 ± 12.39^a

When the enzymatic activity was too low to be quantified, non-detected (ND) is indicated. Values in the same column with different letters are significantly different (p < 0.05)