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Table 2 NaCl concentration effect on the recovery of MPs and PLA2 in PEG–potassium phosphate systems

From: Development of aqueous two-phase systems-based approaches for the selective recovery of metalloproteases and phospholipases A2 toxins from Crotalus molossus nigrescens venom

System identifier System parameters Top phase recovery percentage (%R) Top phase purification factor (PF)
VR TLL (% w/w) NaCl (%w/w) MPs PLA2 Total protein MPs PLA2
S37 0.33 15 1 53.83 ± 16.68a,b 180.54 ± 6.23a 50.37 ± 2.76a 2.14 ± 0.67a,b 143.72 ± 3.12a,b
S38 3 53.19 ± 15.25a,b 141.25 ± 5.64a,b 40.17 ± 2.11b 2.70 ± 0.85a,b 141.64 ± 10.62a,b,c
S39 5 46.25 ± 9.87a,b,c,d 55.78 ± 2.74b 30.60 ± 4.16c,d 2.65 ± 1.07a,b 59.43 ± 20.84e,f
S40 25 1 45.40 ± 6.39a,b,c,d 126.91 ± 1.56a,b 43.26 ± 2.94b 2.09 ± 0.23a,b 118.66 ± 9.52a,b,c,d,e
S41 3 51.24 ± 9.42a,b,c 141.88 ± 3.07a,b 40.42 ± 0.66b 2.54 ± 0.49a,b 140.51 ± 4.11a,b,c,d
S42 5 56.43 ± 1.95a,b 66.73 ± 4.40b 33.12 ± 1.74c 3.41 ± 3.41a,b 81.40 ± 8.71d,e
S43 1.00 15 1 ND 113.16 ± 56.77a,b 27.81 ± 1.23c,d,e ND 85.00 ± 42.67b,c,d,e
S44 3 12.52 ± 7.96c,d,e 106.64 ± 53.36a,b 25.32 ± 1.10d,e,f 0.52 ± 0.33a,b 143.27 ± 13.39a,b
S45 5 66.55 ± 10.70a 121.23 ± 3.51a,b 19.58 ± 0.41 f,g 3.79 ± 0.66a 137.83 ± 6.12a,b,c,d
S46 25 1 18.84 ± 131.67b,c,d,e 131.67 ± 16.14a,b 23.51 ± 1.06e,f,g 0.81 ± 0.64a,b 0.67 ± 0.00 f
S47 3 9.73 ± 5.94d,e 100.81 ± 51.67a,b 24.58 ± 0.51d,e,f 0.42 ± 0.26a,b 83.39 ± 42.06c,d,e
S48 5 26.85 ± 20.26a,b,c,d,e 123.20 ± 6.50a,b 17.19 ± 1.04 h 2.30 ± 1.33a,b 154.71 ± 12.39a
  1. When the enzymatic activity was too low to be quantified, non-detected (ND) is indicated. Values in the same column with different letters are significantly different (p < 0.05)