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Table 2 NaCl concentration effect on the recovery of MPs and PLA2 in PEG–potassium phosphate systems

From: Development of aqueous two-phase systems-based approaches for the selective recovery of metalloproteases and phospholipases A2 toxins from Crotalus molossus nigrescens venom

System identifier

System parameters

Top phase recovery percentage (%R)

Top phase purification factor (PF)

VR

TLL (% w/w)

NaCl (%w/w)

MPs

PLA2

Total protein

MPs

PLA2

S37

0.33

15

1

53.83 ± 16.68a,b

180.54 ± 6.23a

50.37 ± 2.76a

2.14 ± 0.67a,b

143.72 ± 3.12a,b

S38

3

53.19 ± 15.25a,b

141.25 ± 5.64a,b

40.17 ± 2.11b

2.70 ± 0.85a,b

141.64 ± 10.62a,b,c

S39

5

46.25 ± 9.87a,b,c,d

55.78 ± 2.74b

30.60 ± 4.16c,d

2.65 ± 1.07a,b

59.43 ± 20.84e,f

S40

25

1

45.40 ± 6.39a,b,c,d

126.91 ± 1.56a,b

43.26 ± 2.94b

2.09 ± 0.23a,b

118.66 ± 9.52a,b,c,d,e

S41

3

51.24 ± 9.42a,b,c

141.88 ± 3.07a,b

40.42 ± 0.66b

2.54 ± 0.49a,b

140.51 ± 4.11a,b,c,d

S42

5

56.43 ± 1.95a,b

66.73 ± 4.40b

33.12 ± 1.74c

3.41 ± 3.41a,b

81.40 ± 8.71d,e

S43

1.00

15

1

ND

113.16 ± 56.77a,b

27.81 ± 1.23c,d,e

ND

85.00 ± 42.67b,c,d,e

S44

3

12.52 ± 7.96c,d,e

106.64 ± 53.36a,b

25.32 ± 1.10d,e,f

0.52 ± 0.33a,b

143.27 ± 13.39a,b

S45

5

66.55 ± 10.70a

121.23 ± 3.51a,b

19.58 ± 0.41 f,g

3.79 ± 0.66a

137.83 ± 6.12a,b,c,d

S46

25

1

18.84 ± 131.67b,c,d,e

131.67 ± 16.14a,b

23.51 ± 1.06e,f,g

0.81 ± 0.64a,b

0.67 ± 0.00 f

S47

3

9.73 ± 5.94d,e

100.81 ± 51.67a,b

24.58 ± 0.51d,e,f

0.42 ± 0.26a,b

83.39 ± 42.06c,d,e

S48

5

26.85 ± 20.26a,b,c,d,e

123.20 ± 6.50a,b

17.19 ± 1.04 h

2.30 ± 1.33a,b

154.71 ± 12.39a

  1. When the enzymatic activity was too low to be quantified, non-detected (ND) is indicated. Values in the same column with different letters are significantly different (p < 0.05)