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Table 3 Effect of pH on the recovery of MPs and PLA2 in PEG–salt systems

From: Development of aqueous two-phase systems-based approaches for the selective recovery of metalloproteases and phospholipases A2 toxins from Crotalus molossus nigrescens venom

System identifier System parameters Top phase recovery percentage (%R) Top phase purification factor (PF)
PEG molecular weight (g mol−1) TLL (% w/w) pH MPs PLA2 Total protein MPs PLA2
S49 0.33 15 7.5 273.49 ± 26.23d,e 31.18 ± 7.68c,d 93.22 ± 3.9a,b 5.89 ± 0.66a,b,c,d,e,f,g 6.83 ± 1.83b,c
S50 8.0 189.54 ± 21.07e,f,g,h 5.61 ± 2.86e,f 100.00 ± 3.72a 3.73 ± 0.50d,e,f,g 1.11 ± 0.58c
S51 8.5 180.78 ± 6.33e,f,g,h 1.87 ± 3.74e,f 62.15 ± 9.33b,c,d 6.06 ± 0.82a,b,c,d,e,f 1.06 ± 0.53c
S52 9.0 222.20 ± 14.62e,f,g,h 3.74 ± 2.03e,f 49.02 ± 2.72d 9.11 ± 0.75a 1.06 ± 0.86c
S53 9.5 378.24 ± 0.94b,c,d ND 100.00 ± 6.32a 8.62 ± 0.20a,b ND
S54 10.0 257.61 ± 15.97d,e,f 1.06 ± 0.53e,f 100.00 ± 8.32a 4.05 ± 0.11d,e,f,g 0.16 ± 0.08c
S55 25 7.5 277.70 ± 29.78c,d,e 48.33 ± 14.19c 82.79 ± 2.48b,c 6.69 ± 0.63a,b,c,d,e 11.70 ± 3.41b
S56 8.0 152.50 ± 5.45e,f,g,h ND 100.00 ± 10.41a 2.82 ± 0.15f,g,h ND
S57 8.5 126.93 ± 4.62 g,h 2.49 ± 0.31e,f 55.47 ± 11.18c,d 5.05 ± 1.17c,d,e,f,g 1.03 ± 0.34c
S58 9.0 131.34 ± 60.01f,g,h ND 57.87 ± 8.18c,d 5.27 ± 2.59b,c,d,e,f,g ND
S59 9.5 402.92 ± 19.49a,b,c ND 100.00 ± 3.69a 6.88 ± 0.25a,b,c,d ND
S60 10.0 213.77 ± 7.67e,f,g,h ND 100.00 ± 10.76a 3.41 ± 0.34e,f,g,h ND
S61 1 15 7.5 421.10 ± 52.34a,b 362.89 ± 4.50b 46.45 ± 3.45d 8.22 ± 0.68a,b,c 71.91 ± 5.92a
S62 8.0 219.28 ± 37.42e,f,g,h 10.29 ± 3.14e,f 87.40 ± 31.13a,b,c 2.89 ± 1.16f,g,h 1.49 ± 0.82c
S63 8.5 136.16 ± 8.32f,g,h 3.74 ± 3.06e,f 37.12 ± 3.47d 3.70 ± 0.26d,e,f,g 0.70 ± 0.70c
S64 9.0 172.32 ± 80.27e,f,g,h 14.73 ± 12.03d,e,f 49.62 ± 4.79d 3.19 ± 1.51f,g,h 1.92 ± 1.92c
S65 9.5 508.93 ± 25.41a 8.42 ± 6.87e,f 100.55 ± 2.85a 5.06 ± 0.12c,d,e,f,g 0.54 ± 0.54c
S66 10.0 239.52 ± 5.12e,f,g ND 100.00 ± 9.10a 2.49 ± 0.16 g,h ND
S67 25 7.5 457.04 ± 67.24a,b 386.90 ± 2.99a 47.49 ± 3.37d 8.14 ± 1.37a,b,c 68.65 ± 5.36a
S68 8.0 196.51 ± 21.30e,f,g,h 19.21 ± 6.54d,e 49.48 ± 1.75d 3.60 ± 0.28d,e,f,g 1.49 ± 1.17c
S69 8.5 131.16 ± 19.35f,g,h 5.14 ± 3.06e,f 32.00 ± 1.60d,e 3.75 ± 0.62d,e,f,g 1.02 ± 1.02c
S70 9.0 106.36 ± 74.35 h 11.55 ± 9.43e,f 44.86 ± 8.57d 2.76 ± 2.16f,g,h 2.30 ± 2.30c
S71 9.5 ND ND ND ND ND
S72 10.0 275.38 ± 15.76c,d,e ND 100.00 ± 12.35a 3.08 ± 0.48f,g,h ND
  1. When the enzymatic activity was too low to be quantified, non-detected (ND) is indicated. Values in the same column with different letters are significantly different (p < 0.05)