Development of aqueous two-phase systems-based approaches for the selective recovery of metalloproteases and phospholipases A2 toxins from Crotalus molossus nigrescens venom

Enriquez-Ochoa, Daniela; Meléndez-Martínez, David; Aguilar-Yáñez, José Manuel; Licona-Cassani, Cuauhtemoc; Mayolo-Deloisa, Karla

doi:10.1186/s40643-021-00487-y

Bioresources and Bioprocessing

Table 3 Effect of pH on the recovery of MPs and PLA₂ in PEG–salt systems

From: Development of aqueous two-phase systems-based approaches for the selective recovery of metalloproteases and phospholipases A₂ toxins from Crotalus molossus nigrescens venom

System identifier	System parameters			Top phase recovery percentage (%R)			Top phase purification factor (PF)
System identifier	PEG molecular weight (g mol⁻¹)	TLL (% w/w)	pH	MPs	PLA₂	Total protein	MPs	PLA₂
S49	0.33	15	7.5	273.49 ± 26.23^d,e	31.18 ± 7.68^c,d	93.22 ± 3.9^a,b	5.89 ± 0.66^{a,b,c,d,e,f,g}	6.83 ± 1.83^b,c
S50			8.0	189.54 ± 21.07^e,f,g,h	5.61 ± 2.86^e,f	100.00 ± 3.72^a	3.73 ± 0.50^d,e,f,g	1.11 ± 0.58^c
S51			8.5	180.78 ± 6.33^e,f,g,h	1.87 ± 3.74^e,f	62.15 ± 9.33^b,c,d	6.06 ± 0.82^a,b,c,d,e,f	1.06 ± 0.53^c
S52			9.0	222.20 ± 14.62^e,f,g,h	3.74 ± 2.03^e,f	49.02 ± 2.72^d	9.11 ± 0.75^a	1.06 ± 0.86^c
S53			9.5	378.24 ± 0.94^b,c,d	ND	100.00 ± 6.32^a	8.62 ± 0.20^a,b	ND
S54			10.0	257.61 ± 15.97^d,e,f	1.06 ± 0.53^e,f	100.00 ± 8.32^a	4.05 ± 0.11^d,e,f,g	0.16 ± 0.08^c
S55		25	7.5	277.70 ± 29.78^c,d,e	48.33 ± 14.19^c	82.79 ± 2.48^b,c	6.69 ± 0.63^a,b,c,d,e	11.70 ± 3.41^b
S56			8.0	152.50 ± 5.45^e,f,g,h	ND	100.00 ± 10.41^a	2.82 ± 0.15^f,g,h	ND
S57			8.5	126.93 ± 4.62^g,h	2.49 ± 0.31^e,f	55.47 ± 11.18^c,d	5.05 ± 1.17^c,d,e,f,g	1.03 ± 0.34^c
S58			9.0	131.34 ± 60.01^f,g,h	ND	57.87 ± 8.18^c,d	5.27 ± 2.59^b,c,d,e,f,g	ND
S59			9.5	402.92 ± 19.49^a,b,c	ND	100.00 ± 3.69^a	6.88 ± 0.25^a,b,c,d	ND
S60			10.0	213.77 ± 7.67^e,f,g,h	ND	100.00 ± 10.76^a	3.41 ± 0.34^e,f,g,h	ND
S61	1	15	7.5	421.10 ± 52.34^a,b	362.89 ± 4.50^b	46.45 ± 3.45^d	8.22 ± 0.68^a,b,c	71.91 ± 5.92^a
S62			8.0	219.28 ± 37.42^e,f,g,h	10.29 ± 3.14^e,f	87.40 ± 31.13^a,b,c	2.89 ± 1.16^f,g,h	1.49 ± 0.82^c
S63			8.5	136.16 ± 8.32^f,g,h	3.74 ± 3.06^e,f	37.12 ± 3.47^d	3.70 ± 0.26^d,e,f,g	0.70 ± 0.70^c
S64			9.0	172.32 ± 80.27^e,f,g,h	14.73 ± 12.03^d,e,f	49.62 ± 4.79^d	3.19 ± 1.51^f,g,h	1.92 ± 1.92^c
S65			9.5	508.93 ± 25.41^a	8.42 ± 6.87^e,f	100.55 ± 2.85^a	5.06 ± 0.12^c,d,e,f,g	0.54 ± 0.54^c
S66			10.0	239.52 ± 5.12^e,f,g	ND	100.00 ± 9.10^a	2.49 ± 0.16^g,h	ND
S67		25	7.5	457.04 ± 67.24^a,b	386.90 ± 2.99^a	47.49 ± 3.37^d	8.14 ± 1.37^a,b,c	68.65 ± 5.36^a
S68			8.0	196.51 ± 21.30^e,f,g,h	19.21 ± 6.54^d,e	49.48 ± 1.75^d	3.60 ± 0.28^d,e,f,g	1.49 ± 1.17^c
S69			8.5	131.16 ± 19.35^f,g,h	5.14 ± 3.06^e,f	32.00 ± 1.60^d,e	3.75 ± 0.62^d,e,f,g	1.02 ± 1.02^c
S70			9.0	106.36 ± 74.35^h	11.55 ± 9.43^e,f	44.86 ± 8.57^d	2.76 ± 2.16^f,g,h	2.30 ± 2.30^c
S71			9.5	ND	ND	ND	ND	ND
S72			10.0	275.38 ± 15.76^c,d,e	ND	100.00 ± 12.35^a	3.08 ± 0.48^f,g,h	ND

When the enzymatic activity was too low to be quantified, non-detected (ND) is indicated. Values in the same column with different letters are significantly different (p < 0.05)

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