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Table 4 Recovery of metalloproteases (MPs) and phospholipases A2 (PLA2) using ethanol–potassium phosphate systems

From: Development of aqueous two-phase systems-based approaches for the selective recovery of metalloproteases and phospholipases A2 toxins from Crotalus molossus nigrescens venom

System identifier System parameters Top phase recovery percentage (%R) Top phase purification factor (PF)
PEG molecular weight (g mol−1) Ethanol concentration (% w/w) MPs PLA2 Total protein MPs PLA2
S73 0.33 8.5 20.49 ± 6.87a 128.28 ± 6.13a 39.36 ± 0.78a 1.18 ± 0.38
a
75.54 ± 1.39
a
S74 1 16 50.83 ± 8.94b 519.89 ± 22.77a 20.67 ± 1.93b 2.59 ± 0.60
b
260.89 ± 28.72
a
S75 3 24.25 ND 407.73 ± 206.12a 11.02 ± 0.93c ND 268.08 ± 139.77
a
  1. When the enzymatic activity was too low to be quantified, non-detected (ND) is indicated. Values in the same column with different letters are significantly different (p < 0.05)