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Table 4 Recovery of metalloproteases (MPs) and phospholipases A2 (PLA2) using ethanol–potassium phosphate systems

From: Development of aqueous two-phase systems-based approaches for the selective recovery of metalloproteases and phospholipases A2 toxins from Crotalus molossus nigrescens venom

System identifier

System parameters

Top phase recovery percentage (%R)

Top phase purification factor (PF)

PEG molecular weight (g mol−1)

Ethanol concentration (% w/w)

MPs

PLA2

Total protein

MPs

PLA2

S73

0.33

8.5

20.49 ± 6.87a

128.28 ± 6.13a

39.36 ± 0.78a

1.18 ± 0.38

a

75.54 ± 1.39

a

S74

1

16

50.83 ± 8.94b

519.89 ± 22.77a

20.67 ± 1.93b

2.59 ± 0.60

b

260.89 ± 28.72

a

S75

3

24.25

ND

407.73 ± 206.12a

11.02 ± 0.93c

ND

268.08 ± 139.77

a

  1. When the enzymatic activity was too low to be quantified, non-detected (ND) is indicated. Values in the same column with different letters are significantly different (p < 0.05)