Fig. 4

Overexpressing G6PD downregulated expression of NapA but did not interfere with its nuclear localization. Fresh conidia (1 × 10⁵) of N_Gfp (NapA::GFP) and nP.gsdA/N_Gfp (replacing gsdA promoter with niaD promoter in N_Gfp) was incubated in NO₃⁻-MM for 10 h and was then exposed to 0 or 2 mM H₂O₂ for 20 min; nuclei were stained with Hoechst 33258 for 15 min. Scale bar, 20 μm. Images were captured using a laser confocal microscope