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Table 1 Setup of the bioassay multi-well plates

From: Ozonized biochar filtrate effects on the growth of Pseudomonas putida and cyanobacteria Synechococcus elongatus PCC 7942

 

1

2

3

4

5

6

A

1500 µL cells + 300 ppm (non-ozonized)

1500 µL cells + 150 ppm (non-ozonized)

1500 µL cells + 75 ppm (non-ozonized)

1500 µL cells + 25 ppm (non-ozonized)

1500 µL cells + 10 ppm (non-ozonized)

1500 µL cells + 2 ppm (non-ozonized)

B

1500 µL cells + 300 ppm (dry-ozonized)

1500 µL cells + 150 ppm (dry-ozonized)

1500 µL cells + 75 ppm (dry-ozonized)

1500 µL cells + 25 ppm (dry-ozonized)

1500 µL cells + 10 ppm (dry-ozonized)

1500 µL cells + 2 ppm (dry-ozonized)

C

1500 µL cells + 300 ppm (wet-ozonized)

1500 µL cells + 150 ppm (wet-ozonized)

1500 µL cells + 75 ppm (wet-ozonized)

1500 µL cells + 25 ppm (wet-ozonized)

1500 µL cells + 10 ppm (wet-ozonized)

1500 µL cells + 2 ppm (wet-ozonized)

D

  

Blank No cells 1500 µL LB/BG11 + 1000 µL milli-Q

Blank No cells 1500 µL LB/BG11 + 1000 µL milli-Q

0 ppm DOC 1000 µL milli-Q + 1500 µL cells

0 ppm DOC 1000 µL milli-Q + 1500 µL cells

  1. The first three rows show the DOC concentrations (ppm) of the biochar filtrates from low to high concentration (right to left). The filtrates from the non-ozonized biochar, dry-ozonized biochar, and wet-ozonized are represented in row A, B, and C, respectively. The wells labeled “0 ppm DOC” are the controls with no DOC, and the wells labeled “Blank” contain the growth medium without cells