Fig. 5From: Structural diversity of marine anti-freezing proteins, properties and potential applications: a reviewIce-affinity purification of IBPs using an ice-shell (Marshall et al. 2016). Apparatus used to capture IBPs from diverse mixtures of proteins and other solutes. In this system, the separating surface area was improved by isolating the IBPs into an ice shell formed within a rotating round-bottom flask, partially immersed in the sub-zero bath. In general, each ice-binding compound can be recovered from the liquid solutionBack to article page