Table 3 AFPs used for cryopreservation of biological samples

Ocean pout (Macrozoarces americanus)

─

Rabbit parietal cells

0.1 to 20 mg/ml

Ca permeability

(Negulescu et al. 1992)

Fish

─

Equine embryos

20 mg/ml

Reducing cell damage

(Lagneaux et al. 1997)

Antarctic fishes

AFGP

Mouse embryos

40 mg/ml

Post thaw viability

(Rubinsky et al. 1992)

Fish

AFGP

Rat cardiomyocytes

0.5 to 10 mg/ml

Promote intracellular freezing

(Mugnano et al. 1995)

Winter flounder (Pseudopleuronectes americanus)

I

Human RBCs

5 to 160 µg/ml

Influence on ice crystal growth

(Carpenter and Hansen 1992)

Fish

I

Rat liver

1 mg/ml

Preventing ice formation

(Soltys et al. 2001)

Sub-arctic species winter flounder

I

Seabream (Sparus aurata) embryos

20 nL of 10 mg/ml

Improve chilling resistance

(Robles et al. 2007)

Winter flounder

I

Rat hippocampal slice

cultures

10 mg/ml

Protects neurons

from hypothermia/re-warming injury

(Rubinsky et al. 2010)

North Atlantic notothenioid fish (Marcrozoarces americanus)

III

Buffalo bull sperm

0.01, 0.1, 1, and 10 mg/ml

Improving the progressive motility and plasma membrane integrity

(Qadeer et al. 2014)

Notched-fin eelpout (Zoarces elongatus Kner)

III

Human HepG2

2 to 10 mg/ml

Improving cell viability

(Hirano et al. 2008)

Ocean pout (Macrozoarces americanus)

III

Chimpanzee (pan troglodytes) spermatozoa

1, 10, and 100 µg/ml

Improves post-thaw sperm motility

(Younis and Gould 1998)

Winter flounder

I

Bovine oocyte

20 mg/ml

Reducing the leakiness of the cell membranes

(Rubinsky et al. 1991)

Sea raven

II

Ocean pout

III

Diatom (Navicula glaciei)

NgIBP

Human RBCs

25, 50, and 77 µg/ml

Reducing cell damage

(Kang and Raymond 2004)

Winter flounder (Pseudopleuronectes americanus)

I

Human RBCs

0 to 1.54 mg/ml

Inhibition of ice recrystallization

(Chao et al. 1996)

Sea raven (Hemitripterus americanus)

II

Antarctic and Arctic fishes

AFGP

Pig oocytes

0.1, 1, and 40 mg/ml

Protection of membranes at hypothermic temperatures

(Rubinsky et al. 1990)

Winter flounder

I

Sheep embryo

1 or 10 mg/ml

Enhancing store time

(Baguisi et al. 1997)

Ocean pout

III

Liopsetta pinnifasciata

I

Rat insulin cells

10 mg/ml

Prolonging cell lifetime

(Kamijima et al. 2013)

Hypomesus japonicus

II

Zoarces elongates Kner

III

Eleginus gracilis

AFGP