Table 4 Enhancement of curcumin production by metabolic engineering
From: Biotechnological advances for improving natural pigment production: a state-of-the-art review
Hosts | Strategies | Substrate | Yield | Year | References |
|---|---|---|---|---|---|
Curcuma longa | In vitro propagation | / | 260 μg/g fresh weight | 2012 | Pistelli et al. (2012) |
Curcuma aromatica | In vitro propagation | / | 534 μg/g | 2015 | Wu et al. (2015) |
Curcuma longa | Chitosan elicitation | / | 1.3Â mg/g DCW | 2016 | Sathiyabama et al. (2016) |
Curcuma longa | Bacillus endophyticus TSH42 and Bacillus cereus TSH77 were used for bacterization of rhizome in C. longa | / | 4.16Â g/100Â g | 2017 | Chauhan et al. (2017) |
E. coli | Introduction of 4CL, acetyl-CoA carboxylase (ACC) and CUS | Ferulic acid | 57Â mg/L | 2008 | Katsuyama et al. (2008) |
E. coli | Co-expression of TAL, C3H, COMT, 4CL, DCS and CURS1 | Tyrosine | 0.2Â mg/L | 2015 | Rodrigues et al. (2015) |
E. coli | Co-expression of TAL, C3H, COMT, 4CL, and CUS | Tyrosine | 0.67Â mg/L | 2015 | Wang et al. (2015) |
E. coli | Optimization of PBS; employment of heat shock promoters | Ferulic acid | 17 μM | 2017 | Rodrigues et al. (2017) |
E. coli | Optimization of cultivation conditions, including E. coli subspecies, induction parameters, culture media and carbon source concentration | Ferulic acid | 817.7 μM | 2017 | Couto et al. (2017) |
E. coli | Screening a library of 5’-UTR sequence mutants via MAGE | Glucose | 3.8 mg/L | 2018 | Kang et al. (2018) |
Aspergillus oryzae | Overexpression of CUS; Strengthening malonyl-CoA supply via disruption of SnfA and SCAP | Feruloyl-N-acetylcysteamine | 404 μg/plate | 2019 | Kan et al. (2019) |
E. coli | Gene expression optimization via replacement of plasmids; Co-culture system | Tyrosine | 15.9Â mg/L | 2020 | Rodrigues et al. (2020) |
E. coli | Direction evolution of CUS, and cell membrane engineering via overexpression of monoglucosyldiacylglycerol synthase and supplementation of unsaturated fatty acid | 0.6Â g/L palmitoleic acid and 4Â mM ferulic acid | 1.46Â mM curcumin | 2020 | Wu et al. (2020a) |