Fig. 1
From: Economic purification of recombinant uricase by artificial oil bodies
SDS-PAGE of UC chimaera expressed in E. coli with 1 mM IPTG and overnight incubation at 20 °C. Soluble (1) and insoluble (2) fractions before IPTG induction and soluble (3) and insoluble (4) fractions after induction were resolved on SDS-PAGE. Lane 5 is a dilution of lane 3. The positions of intein–caleosin (~ 40 kDa) and uricase (~ 34 kDa) were indicated by a and b, respectively