Strain/plasmid/primers | Characteristic/sequence (5'–3') | Source |
---|---|---|
Strain | ||
E. coli DH5ɑ | Clone host strain | Transgen |
C. glutamicum S9114 | Industrial strain for glutamate production | |
SO26 | l-Ornithine producing strain derived from C. glutamicum S9114 | (Zhang et al. 2019) |
SO30 | SO26 with pfkB1 deletion | This study |
SO31 | SO26 with pfkB2 deletion | This study |
SO32 | SO26 with pfkB1 deletion and pfkB2 deletion | This study |
Plasmid | ||
pK18mobsacB | Mobilizable vector, allows for selection of double-crossover in C. glutamicum, KmR, sacB | Lab stock |
pK18−△pfkB1 | A derivative of pK18mobsacB, harboring △pfkB1 fragment | This study |
pK18−△pfkB2 | A derivative of pK18mobsacB, harboring △pfkB2 fragment | This study |
Primers | ||
pfkB1-up-F | aacgacggccagtgccaagcttTCAGCGAGGTTAAGCATGGTC | This study |
pfkB1-up-R | GCGTGGAATCAATGTGATGATCATGGGGTTACC | This study |
pfkB1-down-F | CATGATCATCACATTGATTCCACGCTGTCGCTCG | This study |
pfkB1-down-R | cggtacccggggatcctctagaCAGCTCCAACGGTGGATACAAC | This study |
pfkB1-check-F | ACATTCACCCCAAACCCGAGT | This study |
pfkB2-up-F | aacgacggccagtgccaagcttGATTGCCTCATTAACGGCAGT | This study |
pfkB2-up-R | CTGCCGGATTTTGTTCCGTCAAGCTCATTGGTGCTC | This study |
pfkB2-down-F | ATGAGCTTGACGGAACAAAATCCGGCAGGTTTCC | This study |
pfkB2-down-R | cggtacccggggatcctctagaAATTCACGCCCTGCTTACCAG | This study |
pfkB2-check-F | GCACCATCGAAATTGGCGAAG | This study |