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Table 1 Strains, plasmids, and primers used in this study

From: Enhanced l-ornithine production from glucose and sucrose via manipulation of the fructose metabolic pathway in Corynebacterium glutamicum

Strain/plasmid/primers

Characteristic/sequence (5'–3')

Source

Strain

  

 E. coli DH5ɑ

Clone host strain

Transgen

 C. glutamicum S9114

Industrial strain for glutamate production

 

 SO26

l-Ornithine producing strain derived from C. glutamicum S9114

(Zhang et al. 2019)

 SO30

SO26 with pfkB1 deletion

This study

 SO31

SO26 with pfkB2 deletion

This study

 SO32

SO26 with pfkB1 deletion and pfkB2 deletion

This study

Plasmid

 pK18mobsacB

Mobilizable vector, allows for selection of double-crossover in C. glutamicum, KmR, sacB

Lab stock

pK18−pfkB1

A derivative of pK18mobsacB, harboring pfkB1 fragment

This study

pK18−pfkB2

A derivative of pK18mobsacB, harboring pfkB2 fragment

This study

Primers

 pfkB1-up-F

aacgacggccagtgccaagcttTCAGCGAGGTTAAGCATGGTC

This study

 pfkB1-up-R

GCGTGGAATCAATGTGATGATCATGGGGTTACC

This study

 pfkB1-down-F

CATGATCATCACATTGATTCCACGCTGTCGCTCG

This study

 pfkB1-down-R

cggtacccggggatcctctagaCAGCTCCAACGGTGGATACAAC

This study

 pfkB1-check-F

ACATTCACCCCAAACCCGAGT

This study

 pfkB2-up-F

aacgacggccagtgccaagcttGATTGCCTCATTAACGGCAGT

This study

 pfkB2-up-R

CTGCCGGATTTTGTTCCGTCAAGCTCATTGGTGCTC

This study

 pfkB2-down-F

ATGAGCTTGACGGAACAAAATCCGGCAGGTTTCC

This study

 pfkB2-down-R

cggtacccggggatcctctagaAATTCACGCCCTGCTTACCAG

This study

 pfkB2-check-F

GCACCATCGAAATTGGCGAAG

This study

  1. Superscript ‘‘R’’ indicates resistance to the following antibiotics: Km kanamycin