Enhanced l-ornithine production from glucose and sucrose via manipulation of the fructose metabolic pathway in Corynebacterium glutamicum

Bioresources and Bioprocessing

Table 2 Engineering C. glutamicum for biobased l-ornithine production driven by glucose and sucrose

Strains (C. glutamicum)	Carbon sources	l-Ornithine accumulation (g/L)	Cell biomass (OD₆₀₀)	l-Ornithine/OD600	Glucose concentration (g/L)	Sucrose concentration (g/L)	Fructose concentration (g/L)
SO26	Glucose	35.88 ± 0.04	11.68 ± 0.43	3.07	21.54 ± 0.09	–	–
	Sucrose	33.96 ± 1.00	12.90 ± 0.22	2.63	2.54 ± 0.01	19.37 ± 0.60	1.62 ± 0.03
	Glucose + sucrose	40.82 ± 0.58	12.91 ± 0.20	3.16	11.43 ± 0.29	20.33 ± 0.47	1.82 ± 0.01
SO30	Glucose	39.08 ± 1.22	14.31 ± 0.33	2.73	–	–	–
SO30	Glucose + sucrose	47.64 ± 0.18	12.77 ± 0.10	3.73	3.59 ± 0.23	21.60 ± 1.17	2.87 ± 0.01
SO31	Glucose	38.85 ± 1.56	13.24 ± 0.46	2.93	–	–	–
SO31	Glucose + sucrose	41.91 ± 0.90	12.69 ± 0.03	3.30	14.09 ± 0.35	19.74 ± 0.46	1.68 ± 0.03
SO32	Glucose + sucrose	32.74 ± 0.21	14.17 ± 0.21	2.31	2.02 ± 0.25	21.05 ± 0.02	11.18 ± 0.27
SO30 (Fed-batch)	Glucose + sucrose	78.0	14.53	5.37	0	13.39	34.23

Fermentations were performed at 250 rpm for 72 h, and the initial sugar concentration was 100 g/L. Results except fed-batch cultivation are the means ± standard deviations in three individual experiments