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Table 1 Dark fermentative biohydrogen production from various substrates (including industrial wastes) using pure culture

From: Microbiomes of biohydrogen production from dark fermentation of industrial wastes: current trends, advanced tools and future outlook

Microorganism

Substrate

Reactor type

Operating conditions

Biohydrogen yield

References

Temperature, T

pH

Clostridium butyricum INET 1

Glucose

Batch

35 °C

Initial = 7.0,

Operation = uncontrolled

2.24 mol H2/mol hexose

Yin and Wang (2017)

Clostridium butyricum INET 1

Xylose

Batch

35 °C

Initial = 7.0,

Operation = uncontrolled

1.23 mol H2/mol hexose

Yin and Wang (2017)

Clostridium butyricum INET 1

Sucrose

Batch

35 °C

Initial = 7.0,

Operation = uncontrolled

1.44 mol H2/mol hexose

Yin and Wang (2017)

Clostridium butyricum INET 1

Lactose

Batch

35 °C

Initial = 7.0,

Operation = uncontrolled

1.83 mol H2/mol hexose

Yin and Wang (2017)

Clostridium butyricum INET 1

Starch

Batch

35 °C

Initial = 7.0,

Operation = uncontrolled

2.17 mol H2/mol hexose

Yin and Wang (2017)

Clostridium butyricum INET 1

Glycerol

Batch

35 °C

Initial = 7.0,

Operation = uncontrolled

0.67 mol H2/mol hexose

Yin and Wang (2017)

Clostridium butyricum TISTR 1032

Food waste

CSTR

37 °C

Initial = 6.0,

Operation = uncontrolled

362 mL H2/g VS

Kanchanasuta et al. (2017)

Clostridium butyricum DSM 10,702

Microalgae

Batch

37 °C

ND

2.74 mol H2/mol glucose

Ortigueira et al. (2015)

Clostridium butyricum CWBI 1009

Glucose

TBSBR

30 °C

Initial = 5.2,

Operation = 5.2

1.67 mol H2/mol glucose

Puhulwella et al. (2014)

Clostridium butyricum

Glycerol

Batch

37 °C

Initial = 7.4,

Operation = uncontrolled

3.0 mol H2/mol glycerol

Kivistö et al. (2013)

Clostridium butyricum CWBI 1009

Glucose

AnSBR

30 °C

Initial = 7.6,

Operation = uncontrolled

2.2 mol H2/mol glucose

Beckers et al. (2013)

Clostridium butyricum CWBI 1009

Glucose

ABR

30 °C

Initial = 8.5,

Operation = uncontrolled

2.49 mol H2/mol glucose

Laurent et al. (2012)

Clostridium butyricum TISTR 1032

Sugarcane bagasse

Serum bottle

37 °C

Initial = 6.5,

Operation = 6.5

1.52 mol H2/mol hexoseused

Plangklang et al. (2012)

Clostridium beijerinckii DSM 791

Rice mill wastewater

Serum bottle

37 °C

Initial = 7.0,

Operation = uncontrolled

214.9 mL H2/L

Rambabu et al. (2021)

Clostridium beijerinckii PS-3

Oil palm sap

Serum bottle

30 °C

Initial = 7.0,

Operation = uncontrolled

141 mL H2/g substrate

Noparat et al. (2012)

Clostridium pasteurianum DSM 525

Glycerol

Serum bottle

37 °C

Initial = 7.0,

Operation = 7.0

1.10 mol H2/mol glycerol

Sarma et al. (2019)

Clostridium pasteurianum

Glucose

Serum bottle

35 °C

Initial = 7.0,

Operation = uncontrolled

2.2 mol H2/mol xylose

Hsieh et al. (2016)

Clostridium BOH3

Fruit waste

Serum bottle

37 °C

Initial = 6.8,

Operation = uncontrolled

359.97 mL H2/g TS utilised

Mahato et al. (2020)

Clostridium thermocellum ATCC 27,405

Date seeds

Serum bottle

50 °C

Initial = 7.0,

Operation = uncontrolled

103.97 mmol H2/ L

Rambabu et al. (2020)

Clostridium acetobutylicum YM1

Rice bran

Batch

34 °C

Initial = 5.5,

Operation = uncontrolled

117.24 mL H2/g sugarconsumed

Azman et al. (2016)

Clostridium thermocellum ATCC 27,405

Sugarcane bagasse

Serum bottle

55 °C

Initial = 6.6,

Operation = uncontrolled

4.89 mmol H2/g mediumadded

Tian et al. (2015)

Clostridium saccharoperbutylacetonicum N1-4

Rice bran

Batch

30 °C

Initial = 6.0,

Operation = uncontrolled

3.37 mol H2/mol sugarconsumed

Dada et al. (2013)

Clostridium tyrobutyricum Fya102

Glucose

CSTR

35 °C

Initial = 6.0,

Operation = 6.0

1.06 mmol H2/mmol glucose

Whang et al. (2011)

Enterobacter aerogenes ZJU1

Aquatic weed

Batch

37 °C

Initial = 6.0,

Operation = uncontrolled

62.2 mL H2/g VS

Song et al. (2020)

Enterobacter asburiae

Lactose

Batch

25.6 °C

Initial = 7.2,

Operation = uncontrolled

1.19 mol H2/mol lactose

Alvarez-Guzmán et al. (2020)

Enterobacter aerogenes CDC 819–56

Sago wastewater

Serum bottle

31 °C

Initial = 6.8,

Operation = uncontrolled

7.42 mmol H2/mol glucose

Ulhiza et al. (2018)

Enterobacter aerogenes PTCC 1221

Rice straw

Serum bottle

37 °C

Initial = 5.8,

Operation = 5.8

19.73 mL H2/g straw

Asadi and Zilouei (2017)

Enterobacter cloacae IIT-BT 08

Distillery effluent

Serum bottle

37 °C

Initial = 7.5,

Operation = uncontrolled

7.38 mol H2/kg CODreduced

Mishra and Das (2014)

Bacillus cereus

Wheat straw

Batch

37 °C

Initial = 7.5,

Operation = uncontrolled

156.4 mL H2/g VS

Saleem et al. (2020)

Ethanoligenens harbinense B49

Glucose

Serum bottle

36 °C

Initial = 6.5,

Operation = uncontrolled

113.5 mmol H2/L

Xu et al. (2016)

Ethanoligenens harbinense YUAN-3

Glucose

Batch

35 °C

Initial = 7.0,

Operation = 4.5

2.62 mol H2/mol glucose

Zhang et al. (2015)

Escherichia coli

Glucose

Serum bottle

37 °C

ND

2.0 mol H2/mol glucose

Bisaillon et al. (2006)

Janthinobacterium agaricidamnosum

Glucose

Serological bottle

25 °C

Initial = 6.5,

Operation = uncontrolled

0.86 mol H2/mol glucose

Alvarado-Cuevas et al. (2015)

Polaromonas jejuensis

Glucose

Serological bottle

25 °C

Initial = 6.5,

Operation = uncontrolled

1.57 mol H2/mol glucose

Alvarado-Cuevas et al. (2015)

Klebsiella pneumoniae

Brewery wastewater

AnBBR

35–36 °C

Initial = 5.5,

Operation = uncontrolled

0.80–1.67 mol H2/mol glucose

Estevam et al. (2018)

Klebsiella pneumoniae BLb01

Glycerol

Batch

39 °C

Initial = 9.0,

Operation = uncontrolled

45.0 mol %

Costa et al. (2011)

  1. ABR: anaerobic biodisc reactor; AnBBR: mechanically stirred anaerobic reactor; AnSBR: anaerobic sequenced-batch reactor; CSTR: continuous stirred tank reactor; TBSBR: trickling-bed sequenced-batch reactor; ND: no data; COD: chemical oxygen demand; TS: total solid; VS: volatile solid