Table 2 Dark fermentative biohydrogen production from industrial wastes using mixed culture
Inoculum source | Substrate | Inoculum pre-treatment | Reactor type | Operating Conditions | % H2 | H2 yield | H2 production rate | Dominant members | Technique for microbial community analysis | References | |
|---|---|---|---|---|---|---|---|---|---|---|---|
Temperature, T | pH | ||||||||||
Wastewater treatment plant | Glycerol | IR | Batch | 36 °C | Initial = 7.0, Operation = uncontrolled | ND | 0.52 mol H2/mol glycerol | ND | ND | ND | Chen et al. (2021) |
Sewage treatment plant | Glucose | IR | Batch | 36 °C | Initial = 7.0, Operation = uncontrolled | ND | ND | ND | Clostridium sensu stricto 1 and Clostridium butyricum | Amplicon sequencing—Illumina MiSeq-PICRUSt | Yin and Wang (2021) |
Biogas slurry | Sake lees | HT, 150 °C, 40 min | Batch | 37 °C | Initial = 6.0, Operation = uncontrolled | ND | 112.07 mL H2/g COD | ND | Pantosa agglomerans, Clostridium acetobutylicum, Clostridium butyricum | Amplicon sequencing—Illumina MiSeq | Choiron et al. (2020) |
Biogas digester of pig farm | Alligator weed | HT, 100 °C, 30 min | Batch | 37 °C | Initial = 7.0, Operation = uncontrolled | ND | 48.4 mL H2/g VS | 5.63 mL H2/g VS h | Clostridium sensu stricto 1, Anaerobacterium, Clostridium IV | Amplicon sequencing—Illumina MiSeq-gene function prediction | Li et al. (2020) |
Chicken manure | Cassava | HT, 95 °C, 15 min | Hungate tube | 36 °C | Initial = 6.0, Operation = uncontrolled | ND | ND | 16.62 mL H2/L h | Clostridiaceae, Porphyromonadaceae and Rimonococcaceae | Amplicon sequencing—Illumina MiSeq | Martinez-Burgos et al. (2020) |
Vinasse effluent | Cassava | HT, 95 °C, 15 min | Hungate tube | 37 °C | Initial = 6.0, Operation = uncontrolled | ND | ND | 21.82 mL H2/L h | Clostridiaceae, Porphyromonadaceae and Rimonococcaceae | Amplicon sequencing—Illumina MiSeq | Martinez-Burgos et al. (2020) |
Indigenous microbes | Banana wastes | ND | Batch | 37 °C | Initial = 7.5, Operation = uncontrolled | ND | 38.08 mL H2 | ND | Lactobacillus and Clostridium | Metagenomic—Illumina HiSeq | Mazareli et al. (2020) |
Indigenous microbes | Coffee wastes | ND | Batch | 30 °C | Initial = 7.0, Operation = uncontrolled | ND | 240 mL H2 | 3040 mL H2/L day | Clostridium sp., Lactobacillus sp., Kazachstania sp. and Saccharomyces sp. | Metagenomic—Illumina NovaSeq | Villa Montoya et al. (2020) |
Indigenous microbes | Sugarcane molasses | ND | AnSTBR-A | 55 °C | Initial = 3.8, Operation = uncontrolled | 51 | 1.18 mol H2/mol total carbohydrates | 88 mL H2/L h | Thermoanaerobacterium | Amplicon sequencing—Ion Torrent | Oliveira et al. (2020) |
Effluent of hydrogen fermenter | Vegetable waste | ND | Batch | 35 °C | Initial = 7.0, Operation = uncontrolled | 43.54 | 151.67 mL H2/g VSadded | ND | ND | ND | Panin et al. (2020) |
Soil sediment of mangroves | Cassava pulp | ND | Serum bottle | 60 °C | Initial = 7.0, Operation = uncontrolled | ND | 23 mL H2/g substrate | ND | Clostridium thermopalmarium, Clostridium isatidis, Thermoanaerobacterium, Fonticella tunisiensis | Amplicon sequencing—Illumina | Pason et al. (2020) |
Municipal wastewater treatment plant | Glycerol | ND | CSTR | 37 °C | Initial = 6.0, Operation = 6.5 | 49 | 403.6 mmol H2/mol Glyconsumed | 49.4 mL H2/L h | Clostridium intestinale | Amplicon sequencing—Illumina MiSeq | Paillet et al. (2019) |
Sewage treatment plant | Glucose | HT, 100 °C, 15 min | Batch | 37 °C | Initial = 7.0, Operation = uncontrolled | ND | 1.4 mol H2/mol glucose | ND | Clostridium sensu stricto 1 (C. butyricum and C. paraputrificum), Paraclostridium, Paeniclostridium and Romboutsia | Amplicon sequencing—Illumina MiSeq | Yang and Wang (2019) |
Buffalo sludge and rumen | Buffalo waste | ND | Batch | 39 °C | Initial = 7.0, Operation = uncontrolled | 48.1 | 120.8 mL H2/g VS | ND | Clostridia Incertae Sedis, Clostridium sensu stricto 1, Prevotella | Amplicon sequencing—Illumina MiSeq | Chiariotti and Crisà (2018) |
Food waste treatment plant | Glucose | No pre-treatment | UASB | 38 °C | Initial = 4.9, Operation = uncontrolled | 43.49 | 0.89 mol H2/mol hexose | 4.68 L H2/L day | Enterobacter cloacae, Aeromonas hydrophila, Clostridium pasteurianum | Amplicon sequencing—Ion Torrent | Cho et al. (2018) |
Mixed culture | Tequila vinasse | ND | CSTR | 35 °C | Initial = 6.5, Operation = 5.8 | 70 | 124 NmL H2/g VSadded | 159 NmL H2/L h | Clostridium beijerinckii, Streptococcus sp. and Acetobacter lovaniensis | Amplicon sequencing—Illumina MiSeq | GarcÃa-Depraect and León-Becerril (2018) |
Soil and composting residue | Sugarcane bargasse | ND | Batch | 37 °C | Initial = 6.0, Operation = uncontrolled | ND | 1.53 mmol H2/L | ND | Clostridium, Bacteroides, Parabacteroides, Porphyromonas, Desulfitobacterium, Bacillus and Methanothermobacter | Metagenomic—Illumina HiSeq | Soares et al. (2018) |
Anaerobic sludge | Food waste | ND | Batch | 35 °C | Initial = 6.3, Operation = 7.0 |  |  |  |  | Metaproteomic | Jia et al. (2017) |
Brewery wastewater treatment plant | Glucose | HT, 90 °C, 30 min | FBR | 37 °C | Initial = 5.5, Operation = 5.5 | ND | 2.3 mol H2/mol glucoseadded | 78 L H2/L day | Clostridium butyricum, Enterococcus sp. and Enterobacter sp. | qPCR | Pugazhendhi et al. (2017) |
Domestic wastewater treatment plant | Glycerol | Ae, 24 h | CSTR | 37 °C | Initial = 5.5, Operation = 5.5 | 61 | 0.58 mol H2/mol glycerol | 88.0 mmol H2/L day | Clostridium sp., Prevotella sp. and Klebsiella sp. | CE-SSCP—Illumina MiSeq | Silva-Illanes et al. (2017) |
Domestic wastewater treatment plant | Glycerol | Ae, 24 h | CSTR | 37 °C | Initial = 6.0, Operation = 6.0 | 57 | 0.26 mol H2/mol glycerol | 51.0 mmol H2/L day | Clostridium sp., Enterococcus sp., Prevotella sp. and Snodgrassella sp. | CE-SSCP—Illumina MiSeq | Silva-Illanes et al. (2017) |
Brewery industry | Food waste | HT, 103–105 °C, 60 min | ASBR | 35 °C | Initial = 5.5, Operation = 5.5 | 37.9 | 103.6 mL H2/g CODremoved | 226.4 mL H2/L h | Megasphera, Veillonella | Pyrosequencing—Roche | Moreno-Andrade et al. (2015) |
Fruit juice wastewater treatment plant | Food waste | HT, 100 °C, 60 min | Batch | 37 °C | Initial = 6.0, Operation = uncontrolled | ND | 2.68 mol H2/mol hexose | ND | Clostridium frigidicarnis | Pyrosequencing—Roche | Laothanachareon et al. (2014) |
Sugarcane stillage treatment plant | Sugarcane stillage | HT, 90 °C, 10 min | AFBR | 55 °C | ND | 43.3–48.9 | 2.23 mmol H2/g CODadded | 1.49 L H2/L h | Megasphera sp., Lactobacillus | PCR-DGGE—Cloning | Santos et al. (2014) |
Garbage compost | Beer lees | No pre-treatment | Batch | 37 °C | Initial = 4.1–7.0, Operation = uncontrolled | ND | 29.3 mL H2/g TS | ND | Clostridium roseum | PCR-DGGE | Bando et al. (2013) |
Municipal wastewater treatment plant | Rice straw hydrolysate | HT, 95–100 °C, 60 min | CSABR | 37 °C | Initial = 5.5, Operation = 5.5 | ND | 0.69 mol H2/mol T-sugar | 10 L H2/L day | Clostridium pasteurianum | PCR-DGGE | Liu et al. (2013) |
Beach | Sucrose | HT, 100 °C, 45 min | AGSBR | 35 °C | Initial = 6.0, Operation = 6.0 | 37 | 1.04 mol H2/mol sucrose | 15.59 m3 H2/ m3 day | Clostridium pasteurianum and Bifidobacteria sp. | PCR-DGGE | Lin et al. (2011) |
Wastewater treatment plant | Corn stover hydrolysate | HT, 100 °C, 60 min | Serum bottle | 37 °C | Initial = 7.0, Operation = uncontrolled | ND | 502 mL H2/L | 37.3 mL H2/h | Clostridium bifermentans, Escherichia coli, Escherichia vulneris, Uncultured Escherichia sp. | PCR-DGGE—Cloning | Zhang et al. (2011) |
Wastewater treatment plant | Corn stover hydrolysate | Ac, pH 3, 60 min | Serum bottle | 37 °C | Initial = 7.0, Operation = uncontrolled | ND | 352.1 mL H2/L | 13.7 mL H2/h | Enterobacter aerogenes, Escherichia coli, Escherichia vulneris, Uncultured Escherichia sp. | PCR-DGGE—Cloning | Zhang et al. (2011) |
Wastewater treatment plant | Corn stover hydrolysate | Ba, pH 12, 60 min | Serum bottle | 37 °C | Initial = 7.0, Operation = uncontrolled | ND | 458.4 mL H2/L | 12.3 mL H2/h | Enterobacter aerogenes, Klebsiella, Pectobacterium sp. | PCR-DGGE—Cloning | Zhang et al. (2011) |
Wastewater treatment plant | Corn stover hydrolysate | Us, 15 min | Serum bottle | 37 °C | Initial = 7.0, Operation = uncontrolled | ND | 295.9 mL H2/L | 18.7 mL H2/h | Enterobacter aerogenes, Pectobacterium sp. | PCR-DGGE—Cloning | Zhang et al. (2011) |
Wastewater treatment plant | Corn stover hydrolysate | Uv, 15 min | Serum bottle | 37 °C | Initial = 7.0, Operation = uncontrolled | ND | 290.9 mL H2/L | 58.9 mL H2/h | Pectobacterium sp. | PCR-DGGE—Cloning | Zhang et al. (2011) |
Sewage treatment plant | Molasses | HT, 100 °C, 45 min | CSTR | 35 °C | Initial = 5.5, Operation = 5.5 | 47 | 2.1 mol H2/mol hexose | 153 mmol H2/L day | Clostridium acetobutylicum and Clostridium pasteurianum | qPCR | Lay et al. (2010) |