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Bioresources and Bioprocessing

Table 1 Substrate specificity of PcMan113

From: Reshaping the binding channel of a novel GH113 family β-mannanase from Paenibacillus cineris (PcMan113) for enhanced activity

Substrate

Relative activitya

(100%)

Specific activityb (u/mg)

LBG

100

31.7 ± 0.13

KG

2.9 × 102

68.2 ± 0.36

GG

8.5

2.1 ± 0.07

SCC

ND

ND

BX

ND

ND

M2

0.9 × 103

2.6 × 102 ± 12.1

M3

2.1 × 104

3.2 × 103 ± 30.8

M4

3.5 × 104

7.5 × 103 ± 65.4

M5

3.9 × 104

8.2 × 103 ± 48.0

M6

3.3 × 104

6.9 × 103 ± 78.6

  1. ND not detected
  2. aThe relative activity of PcMan113 towards LBG was set as 100%
  3. bThe specific activity of substrates by PcMan113 catalyzing in vitro. Reaction conditions: 5 mM substrate with 0.2 mg mL−1 purified protein in 200 μL PBS–citric acid buffer (20 mM, pH 6.0) at 45℃ for 10 min
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