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Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Immobilization of engineered E. coli cells for asymmetric reduction of methyl acetoacetate to methyl-(R)-3-hydroxybutyrate

Fig. 1

Reaction course curve of the asymmetric reduction of MAA to (R)-HBME catalyzed by free, alginate-immobilized and alginate chitosan-immobilized recombinant E. coli cells. Reaction conditions: 4 mL Tris–HCl buffer (200 mM, pH 7.0), 100 mM MAA, 150 mM glucose, 30 mg/mL of free recombinant E. coli cells (wet weight), or alginate-immobilized cells or the alginate chitosan-immobilized cells containing the same amount of free cells, 40 °C, 200 rpm

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