Fig. 4From: Immobilization of engineered E. coli cells for asymmetric reduction of methyl acetoacetate to methyl-(R)-3-hydroxybutyrateReusability of free and alginate-immobilized recombinant E. coli cells. Reaction conditions: 4 mL Tris–HCl buffer (200 mM, pH 7.0), 100 mM MAA, 150 mM glucose, 30 mg/mL of free recombinant E. coli cells (wet weight), or immobilized cells containing the same amount of free cells, 40 ℃, 200 rpm. (n = 3, different lowercase letters represent significant differences between groups, p < 0.05)Back to article page