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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Enhanced thermal and alkaline stability of L-lysine decarboxylase CadA by combining directed evolution and computation-guided virtual screening

Fig. 1

Establishment of the HTS method. a Colorimetric method for the determination of lysine decarboxylase activity. b Color change of thymol blue with pH increase at pH 8.0. c Color change of the mixed indicator with pH increase at pH 8.0. d Correlation between the absorbance at 550 nm and the HPLC results. The reaction was conducted at 50 ºC in borate buffer (pH 8.0), and samples were taken after 0, 15, 30, 45, and 60 min of reaction. The cadaverine concentration was determined by HPLC. The error bars represent standard deviations calculated from triplicate experiments

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