Fig. 2From: Enhanced thermal and alkaline stability of L-lysine decarboxylase CadA by combining directed evolution and computation-guided virtual screeningDirected evolution of CadA. a High-throughput screening of error-prone PCR mutation libraries. b Site-directed mutagenesis of K477. The error bars represent standard deviations calculated from triplicate experiments, and statistical significance of the different relative cadaverine yield in comparison with the wild type was evaluated using Student’s t test (*, P \(<\) 0.05, **, P \(<\) 0.01)Back to article page