From: Enhanced thermal and alkaline stability of L-lysine decarboxylase CadA by combining directed evolution and computation-guided virtual screening
Mutants
Relative yield (%)
Wild Type
100
T88S
105.2
K477R/E445Q
124.1
K477R/F102V
103.2
K477R/T88S
105.5
K477R/E445Q/T88S
93.4
K477R/E445Q/F102V
103.5
K477R/E445Q/T88S/F102V
137.7