From: Enhanced thermal and alkaline stability of L-lysine decarboxylase CadA by combining directed evolution and computation-guided virtual screening
Mutants
Total number of salt bridges
(probability > 5%)
Number of salt bridges
(probability > 80%)
WT
28
7
K477R
25
10
E445Q
29
8
F102V
9
K477R/E445Q
30
K477R/E445Q/T88S/F102V